Evaluation of Membrane Protein Properties by Fluorescence-Detection Size-Exclusion Chromatography (FSEC) Using an HPLC System

Application Note Evaluation of Membrane Protein Properties by Fluorescence-Detection Size-Exclusion Chromatography (FSEC) Using an NexeraTM lite inert system Emiko Ando1、Hidetoshi Terada1 Life Science Life Science  Abstract However, when membrane proteins are transiently expressed using cultured cells, in many cases,…

Cytosolic expression of Green Fluorescent Protein (GFP) and its derivatives in the yeast Saccharomyces cerevisiae: Detection in vivo using the Agilent Cary Eclipse Application Note Author Paul Gavin# and Mark Prescott#, Ph.D Daren J. Fyfe, Ph.D* #Department of Biochemistry and…

Application Note Breeze through membrane protein stability with Uncle Introduction Membrane proteins are some of the most important and common drug targets in medicine. Mutated membrane proteins cause disease and are biomarkers for cancers, viral and bacterial membrane proteins are…

Monitoring fluorescence resonance energy transfer (FRET) between GFP fusions in lysates of the yeast Saccharomyces cerevisiae using the Agilent Cary Eclipse Application Note Author Introduction Paul Gavin* and Mark Prescott*, Ph.D Fluorescence resonance energy transfer (FRET) is a non-destructive, spectroscopic…