Efficient early-phase screening of siRNA purity and impurities using denaturing IP-RPLC-UV with dual-channel UHPLC

Accelerate method scouting for GalNAc-conjugated siRNA candidates

Discover a faster, structured approach to early-phase siRNA purity and impurity screening.

This webinar explores how denaturing ion-pair reversed-phase LC-UV and a dual-channel UHPLC system can streamline method scouting for GalNAc-conjugated siRNA candidates across multiple ion-pairing reagents and stationary phases.

Learn how parallel workflows can complete 320 chromatographic runs in 48 hours, cut instrument time by 50%, reduce manual handling, and generate comprehensive data for method selection. The session will also examine sequence-dependent strand separation and elution behavior that can influence analytical performance and optimization.

Key learnings:

• How to design a comprehensive denaturing IP-RPLC-UV scouting strategy for siRNA purity and impurity assessment.
• How a dual-channel UHPLC workflow can increase throughput while reducing instrument time and manual intervention.
• How to compare six ion-pairing reagents and four stationary phases for sense- and antisense-strand separation.
• How retention-time differences, peak shape, and strand-area trends can help prioritize conditions for further optimization.
• Why ion-pairing reagent performance and strand elution order can be sequence dependent—and why column chemistry may have a secondary influence.

Who should attend

• Analytical scientists developing methods for siRNA and therapeutic oligonucleotides
• LC and UHPLC chromatographers and method-development specialists
• Pharmaceutical analytical R&D and early-phase candidate-screening teams
• Scientists responsible for purity, impurity, and strand-level characterization
• Laboratory and workflow leaders seeking higher-throughput method scouting

Reserve your place and learn how to accelerate siRNA method development while maintaining comprehensive analytical coverage.

If you cannot attend the broadcast, please register anyway to automatically receive the link to the recording.

Presenter: Martina Lioi (Senior Expert Analytical Science, Novartis Pharma AG, Technical Research & Development (TRD), Analytical Research & Development (ARD))

Presenter: Sylvia Grosse (Product Applications Specialist, Thermo Fisher Scientific)

Sylvia Grosse is a Product Applications Specialist in the HPCS division at Thermo Fisher Scientific. Sylvia has over 15 years of laboratory experience. She began her analytical journey in an academic research group working with (U)HPLC and MS/(MS) instruments before joining Thermo Fisher Scientific in 2017. During her career, until now she has published more than 30 scientific publications, application notes, and technical notes for food & beverage, (bio)pharma and environmental markets. Sylvia is focused on improving processes inside and outside the laboratory to make life easier.