Advancing the characterization of mono- and multi-specific antibodies at the intact and subunit levels using IR photon-assisted dissociation methods

Antibody-based therapeutics are becoming increasingly complex, creating new challenges for obtaining detailed sequence and structural information across critical regions of these molecules.

Join Luca Fornelli, PhD, Associate Professor at the University of Oklahoma, as he explores middle-down and native top-down mass spectrometry approaches for the characterization of antibody-based therapeutics using the Thermo Scientific™ Orbitrap™ Tribrid™ Apex BioPharma Mass Spectrometer.

Luca will show how infrared-assisted electron transfer dissociation (IR-ETD) and electron transfer/higher-energy collision dissociation (IR-EThcD) can be combined with proton transfer charge reduction (PTCR) to generate detailed sequence information from complex biologics. The talk will discuss denaturing and native conditions, automated data analysis, Fab fragments, and intact monoclonal, bispecific, and trispecific antibodies.

Who should attend?

Scientists and researchers involved in biopharmaceutical characterization and development, including monoclonal and multispecific antibodies, middle-down and top-down MS, native MS, biotherapeutic sequence and structural characterization, and advanced fragmentation workflows.

What you can expect to learn

• How middle-down and native top-down MS provide complementary information
• How IR-ETD, IR-EThcD, and PTCR can improve fragmentation and simplify spectra
• Approaches for increasing sequence information from disulfide-protected regions and CDRs
• How advanced fragmentation can be applied to subunits, Fab fragments, and intact therapeutics
• How automated data analysis can streamline interpretation

Presenter: Luca Fornelli. PhD (Principal Investigator, University of Oklahoma)

Luca Fornelli earned his PhD in Chemistry from EPFL and completed postdoctoral work in Neil Kelleher’s lab at Northwestern University, developing and applying top-down proteomics methods. He is an Associate Professor of Biology at the University of Oklahoma, where he develops data acquisition strategies for large proteoforms.

Presenter: Kristina Srzentic (Manager, Pharma &BioPharma Applications Development, Thermo Fisher Scientific)

Kristina is an LC-MS scientist with over 10 years of experience in protein mass spectrometry and biopharmaceutical characterization. At Thermo Fisher Scientific, her work spans analytical method development, product management, and scientific marketing, with expertise in Orbitrap mass spectrometry and targeted and high-throughput protein analysis.

Moderator: Breana Murphy (Regional Marketing Manager LSMS-North America, Thermo Fisher Scientific)