Advancing Oligonucleotide Bioanalysis with High-Resolution Mass Spectrometry

High-resolution accurate mass (HRAM) mass spectrometry has transformed quantitative bioanalysis, opening new possibilities for challenging analytes that have historically pushed the limits of conventional approaches. Join Troy Voelker for a practical look at the evolution of HRAM mass spectrometry for quantitative bioanalysis and how advances in Orbitrap™ technology are helping address increasingly complex analytical challenges.

Drawing on more than a decade of experience applying high-resolution mass spectrometry to quantitative workflows, Troy will trace the progression from early studies evaluating whether HRAM could deliver the speed, precision, specificity, and dynamic range required for quantitative analysis to its growing role in modern bioanalysis.

The session explores oligonucleotide bioanalysis, including multiple charge states, nonspecific fragmentation, matrix interference, and demanding sensitivity requirements. Troy will share findings from an industry oligonucleotide ring trial and discuss how established methods provided a benchmark for evaluating the next generation of Orbitrap technology, featuring the new Thermo Scientific™ Orbitrap™ Excedion™ Hybrid Mass Spectrometer.

Attendees will have the opportunity to participate in the live Q&A following the presentation.

Who should attend?

Scientists and laboratory leaders developing quantitative bioanalytical methods using HRAM MS; researchers working with oligonucleotides and nucleic acid therapeutics; and bioanalytical scientists interested in sensitivity, specificity, and quantitative performance.

What you can expect to learn

• How HRAM MS has evolved for quantitative bioanalysis
• Why charge-state behavior and fragment selection are critical for oligonucleotide LC-MS specificity
• How hybridization sample preparation and HRAM detection can support sensitive workflows
• How Orbitrap Excedion technology can extend sensitivity, speed, and dynamic range

Presenter: Min Du (Senior Manager, Pharma/BioPharma Applications, Thermo Fisher Scientific)

Min Du is the Senior Manager of Pharma/Biopharma Applications for Chromatography and Mass Spectrometry at Thermo Fisher Scientific. With extensive expertise in analytical workflows, she leads teams developing cutting-edge LC-MS solutions. Her work focuses on bioanalysis challenges, RNA oligonucleotide characterization, and advancing therapeutic workflows.

Presenter: Troy Voelker (Senior Director of Laboratory Operations, Aliri Bioanalysis)

Troy Voelker is an accomplished bioanalytical scientist with more than a decade of experience advancing high-resolution mass spectrometry. His career spans pioneering quantitative HRMS applications to today’s cutting-edge oligonucleotide bioanalysis, with deep expertise in developing methods for complex therapeutics.

Moderator: Breana Murphy (Regional Marketing Manager LSMS-North America, Thermo Fisher Scientific)