Walk-Up Mass Confirmation for Synthetic Oligonucleotides

Enabling Walk-Up Mass Confirmation for Synthetic Oligonucleotides: Simplifying LC-MS Analysis with ReadyAccess and BioAccord

Oligonucleotide and RNA therapeutics are advancing rapidly, creating new possibilities for addressing complex diseases while increasing the need for robust, accessible analytical workflows. From synthetic oligonucleotides and antisense oligonucleotides to siRNA, mRNA, and CRISPR single-guide RNA, these molecules must be carefully characterized to confirm molecular mass, sequence identity, purity, and critical modifications. Reliable analytical methods are also essential for understanding impurities, improving method robustness, and supporting confident decisions throughout research, development, and quality control.

In this third annual, three-week webinar series, scientists from Waters Corporation and collaborators from Synthon and BioSpring will explore current challenges and practical approaches for oligonucleotide analysis. Topics will include improving the robustness of ion-pair reversed-phase LC-UV-MS methods, expanding access to walk-up mass confirmation for synthetic oligonucleotides, and applying LC-MS oligo mapping and tunable RNase digestion to advanced RNA characterization.

Join us as we explore the science of oligos and the power of LC, LC-MS, informatics, and complementary analytical workflows to characterize them with greater confidence.

Confirming the molecular mass of synthetic oligonucleotides is essential during discovery and development, yet access to LC-MS analysis can be limited by reliance on specialist operators and centralized laboratories.

In this webinar, we explore a walk-up approach using ReadyAccess software with the BioAccord LC-MS System, supported by deconvolution and data management in Empower. Learn how accessible acquisition and automated processing can help oligonucleotide scientists independently confirm molecular mass, assess sample quality, reduce turnaround times, and streamline routine decision-making across research and development laboratories.

Why you should attend

• Understand how walk-up LC-MS can expand access to intact-mass confirmation for synthetic oligonucleotides.
• See how ReadyAccess and BioAccord can simplify sample submission, acquisition, and results review.
• Learn where Empower deconvolution can support oligonucleotide mass confirmation and compliant workflows.
• Identify opportunities to reduce turnaround time and reliance on centralized MS experts.

Who should attend

This webinar is designed for synthetic oligonucleotide chemists, analytical scientists, medicinal chemists, LC-MS laboratory managers, informatics leads, and R&D teams seeking accessible intact-mass confirmation workflows.

Presenter: Heidi Gastall (Principal Product Manager, Process Analytics, Waters Corporation)

Heidi Gastall is a Principal Product Manager at Waters Corporation. She received her Ph.D. from the University of Oxford and holds an MSci and BA (Hons) from the University of Cambridge.

Since joining Waters in 2019, she has held roles in applications chemistry, product ownership, and product management. Her work focuses on developing accessible software and LC-MS solutions for biopharmaceutical scientists, including ReadyAccess, BioAccord, waters_connect, and Empower workflows for synthetic oligonucleotide mass confirmation and analysis.