Processing TMT data with Proteome Discoverer Software: From raw data to reliable quantification

Tandem Mass Tag (TMT) labeling enables powerful multiplexed quantitative proteomics experiments, but obtaining high-quality results requires the right data-processing strategy. From selecting appropriate workflows and quantification settings to evaluating reporter-ion signals and addressing interference, each step can influence the quality and reliability of the final quantitative results.

In this webinar, we will provide a practical guide to processing TMT and TMTpro data using Thermo Scientific Proteome Discoverer software. We will discuss how to prepare for data processing, select workflows according to the instrument and acquisition method, configure TMT quantification, and apply key parameters for reporter-ion quantification and normalization.

The session will also cover approaches for TMTpro 32/35plex experiments and data acquired using the Orbitrap Astral Zoom mass spectrometer. Finally, we will explore useful quality metrics for assessing experimental outcomes and walk through practical troubleshooting examples to illustrate how common quantification issues can be identified.

Join us to learn how to get more from your TMT experiments and build greater confidence in your quantitative proteomics results.

If you are not able to attend, please register and the on demand link with be automatically sent to you.

Presenter: Michaela Scigelova (Product Support, Thermo Fisher Scientific)

Michaela provides customer support to Thermo Scientific Proteome Discoverer software users.