Comparison of biocompatible and inert UHPLC systems for LC-UV quantitation of large RNA

Missed the International Symposium on Chromatography 2026? Explore our posters and talks now.

Comparison of biocompatible and inert UHPLC systems for LC-UV quantitation of large RNA

See how the Thermo Scientific Vanquish Amplify UHPLC system helps reduce metal-analyte interactions for metal-sensitive analytes and large RNA workflows.

Accurate LC-UV analysis of large RNA and oligonucleotide therapeutics requires highly inert chromatographic flow paths to minimize analyte loss, peak broadening, and reduced sensitivity. This webinar is based on a poster presented at the International Symposium on Chromatography 2026 and will share data comparing the Vanquish Amplify UHPLC system with biocompatible and other inert UHPLC configurations for metal-sensitive analytes such as AMPcP and large RNA fragments.

Based on recent poster data from ISC 2026, this session will show how integrated analytics can provide faster, data-driven feedback during mAb and bispecific process development—helping teams optimize productivity without compromising product quality.

What you’ll learn

• How metal surfaces can affect LC-UV performance for phosphodiester-containing analytes
• How the Vanquish Amplify system performed for 500 nt and 5,000 nt RNA fragments
• How the system compared with MP35N-based fluidics and a commercial inert platform under the tested conditions

Key takeaways 

• Up to a twofold sensitivity increase was observed for AMPcP under conditions where metal interactions were most pronounced.
• For large RNA, the Vanquish Amplify UHPLC system delivered 1.25–1.35× higher signal intensity versus systems with MP35N fluidics.
• The inert flow path supported improved chromatographic performance and reproducibility, especially for low-concentration analytes

Who should attend

This webinar is ideal for scientists working in oligonucleotides, RNA analysis, LC-UV quantitation, and method development for metal-sensitive analytes.

Register now to see how inert UHPLC design can improve sensitivity and reliability in large RNA analysis.

If you are unable to attend the live webinar, please register to automatically receive a link to the on-demand recording. 

Presenter: Sukhdev Bangar (Senior Product Marketing Manager, Thermo Fisher Scientific)

Sukhdev graduated from De Montfort University (UK) with a BSc. in Pharmaceutical Sciences. After which he worked in the Pharmaceutical industry at GSK & Astra Zeneca performing LC & LC-MS analyses. From 2005 till 2023 Sukhdev worked various roles at Waters Corporation; LC-MS applications, Pharma LC-MS Demo group leader and HPLC Product Manager. In his current role at Thermo Fisher Scientific he is responsible for marketing activities for the Vanquish analytical LC’s product portfolio. In this role, he represents the voice of the customer helping shape the future of Vanquish LC systems.