Polymeric, porous, monodisperse particle development and application to reversed-phase separations of biological molecules

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Polymeric, porous, monodisperse particle development and application to reversed-phase separations of biological molecules

Join us for a webinar exploring the development of polymeric, monodisperse supermacroporous particles for reversed-phase separations of biological molecules. As oligonucleotides, RNA, proteins, and other biomolecules increase in size and complexity, stationary-phase design becomes increasingly important for achieving effective retention, recovery, and resolution.

This session will focus on how particle architecture influences chromatographic performance, including the effects of particle-size distribution, pore diameter, surface area, pore morphology, and surface chemistry. The presentation will highlight the development of approximately 2.5 µm monodisperse particles with reduced fines, the relationship between surface area and pore accessibility, and separation examples across a wide polynucleotide size range, including ssDNA, dsDNA, siRNA, sgRNA, and mRNA. The webinar will also discuss how incorporating tertiary amine comonomers can increase oligonucleotide retention and improve separations using mass spectrometry-compatible, ion-pairing-agent-free mobile phases.

What you will learn

Attendees will gain insight into:

• How monodisperse particle technology can enable smaller particle sizes while minimizing fines
• Why pore size, pore shape, and accessible surface area are critical for larger biomolecule separations 
• How surface area affects retention and resolution across short and long polynucleotide samples 
• How supermacroporous particle design supports separations of siRNA, sgRNA, and mRNA 
• How amine comonomer chemistry can be used to modify retention and separation behavior 

Who should attend

This webinar is intended for analytical scientists, LC method developers, separation scientists, biopharma researchers, and nucleic acid therapeutics teams working with oligonucleotides, RNA, proteins, or other complex biomolecules.

Why attend

This webinar provides a particle-development and separation-mechanism perspective. Attend this session to better understand the stationary-phase design principles that can influence reversed-phase biomolecule separation performance.

Cannot attend the webinar broadcast? Please register anyway to automatically receive the link to recording.

Presenter: Shane Bechler (Sr. R&D Manager - Column Engineering, Thermo Fisher Scientific)

Dr. Shane Bechler is a Senior Manager of Research and Development at Thermo Fisher Scientific, with over a decade of experience in advancing biotechnological and chemical engineering innovations. He holds a Ph.D. in Chemical Engineering from the University of Wisconsin-Madison and a B.S. from the Georgia Institute of Technology.