Integration of Automated Sampling with LC-MS as a PAT Tool for USP Glycosylation Monitoring and Control

In this webinar, you will learn:

• Current limitations in conventional sampling and analytical methods
• The role of automated sampling in enhancing process control
• How digital technologies and PAT are integrated to drive bioprocess innovation
• About case studies for on-line glycosylation monitoring

Detailed description:

This webinar explores how automation strategies and control schemes facilitate rapid analytical results, crucial for managing glycosylation variability that affects product quality. The MAST® Autosampling Solution and Agilent 2D-LC/TOF MS was used to enable glycosylation monitoring during perfusion cell culture. A digital lab infrastructure and cloud-based orchestration layer managed communication and control between the units, allowing easy modification of parameters like target galactosylation and sampling frequency. Over a 35-day culture process, targeted galactosylation setpoints were maintained within a ± 2% deadband, ensuring efficient data collection and timely insights. 

An on-demand version of this webinar will be available after the live event using the same link. Register now and access the webinar at your convenience.

Presenter: Srijana Chapagain (PAT & Digital Applications)

Srijana Chapagain is an Applications Engineer within the Single Use and Integrated Systems PAT & Digital Applications Team. She joined our company in 2020 and was previously a part of the SU&IS Upstream Applications Team.

Srijana holds a B.Sc. in Chemical Engineering from Villanova University and a M.Sc. in Engineering Management from Tufts University. In her role, Srijana has been involved in various product development initiatives and technology assessments within the Upstream Bioprocessing and Process Analytical Technology (PAT) -enabling sector. Her current focus encompasses PAT integration and the digital transformation of laboratory operations at her site.