LC Column Troubleshooting – Isolating the Source of the Problem

Troubleshooting an HPLC system can be a daunting task. In this seminar we will:

• Look at how to isolate the problem

• Discuss the most common column problems and their solutions including:

  • Pressure

  • Peak Shape

  • Retention

We will also look at examples where you may think it’s your column but it’s actually related to your instrument such as:

• Dwell volume
• Extra Column Volume
• Detector Response Rate

Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.