Entering the Bio Zone: Reversed Phase HPLC from Small Molecules to Biomolecules

Whether you’re starting out working on biomolecules or transitioning from small molecules to biotherapeutics you will find some attributes can make biomolecule separations more challenging.

This presentation will look at what we typically think about for small molecules and how working with biomolecules may be similar or in some cases quite different.

We’ll discuss:

• Method development parameters; similarities and differences

• Column selection

• The matrix and sample prep

• Instrument considerations

• Tips to help identify why your separation doesn’t look like you thought it would

• Although the focus of this talk is reversed phase, we will also review other types of chromatography used for biomolecules including HIC, SEC, and IEX.

Presenter: Rita Steed (Application Engineer, Agilent Technologies, Inc.)

Rita Steed began supporting the LC column line for Agilent in 1999. As a Chromatography Specialist (first with Chromatography, Inc., then directly for Agilent), Rita worked on-site with researchers in Pharmaceutical and other industries presenting technical seminars and assisting researchers with troubleshooting and method development. Rita has over 20 years of Chromatography experience in the Biotechnology, Chemical, and Pharmaceutical industries. She has held positions in Research, Sales, and Technical Service. Rita has earned degrees in Microbiology and Life Sciences/Biochemistry. In her current position, she is an inside Application Engineer supporting LC columns.