Shim-pack Velox LC Columns - Maximize LC Separation Performance with Core Shell Technology

Shim-pack V

elox LC Columns

C190-E219

Maximize LC Separation Performance with Core Shell Technology 

Shim-pack Velox LC Columns

© Shimadzu Corporation, 2018

www.shimadzu.com/an/

For Research Use Only. Not for use in diagnostic procedures. 

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does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

First Edition: September 2018, Printed in Japan 3655-08801-20AIK

Free-turning architecture lets you change cartridges by hand without breaking inlet / outlet fluid connections — no tools needed.
Guard column cartridges require Shim-pack Velox EXP Direct Connect Holder (227-32041-01)

Shim-pack Velox EXP Guard Column

Tubing

Nut

Ferrule

Peek End

Ferrule

Peek End

Holder Cap

Holder Body

Guard Column

Cartridge

Ordering Information

Shim-pack Velox 5 µm Columns

Shim-pack Velox EXP Guard Column Cartridge (3 pk)

Shim-pack Velox UHPLC Precolumn Filter (0.2 µ

ｍ)

＊Shim-pack Velox EXP Direct Connect Holder : 227-32041-01

Chemistry

50

100
150
250

SP-C18

227-32006-01
227-32006-02
227-32006-03
227-32006-04

C18

227-32012-01
227-32012-02
227-32012-03
227-32012-04

Biphenyl

227-32018-01
227-32018-02
227-32018-03
227-32018-04

4.6 

PFPP

227-32024-01
227-32024-02
227-32024-03
227-32024-04

Type

ID (mm)

2.1
3.0
4.6

UHPLC

SP-C18

227-32028-01
227-32028-02

–

C18

227-32031-01
227-32031-02

–

Biphenyl

227-32034-01
227-32034-02

–

PFPP

227-32037-01
227-32037-02

–

SP-C18

227-32029-01
227-32029-02
227-32029-03

2.7 µ

ｍ

C18

227-32032-01
227-32032-02
227-32032-03

Biphenyl

227-32035-01
227-32035-02
227-32035-03

PFPP

227-32038-01
227-32038-02
227-32038-03

HILIC

227-32040-01
227-32040-02
227-32040-03

Type

ID (mm)

4.6

5 µ

ｍ

SP-C18

227-32030-01

C18

227-32033-01

Biphenyl

227-32036-01

PFPP

227-32039-01

Part No. 1 pack
Part No. 5 pack
Part No. 10 pack

227-32042-01
227-32042-02
227-32042-03

To minimize extra column volume and maximize UHPLC sample throughput with SPE, 
SLE, or other sample preparation techniques, pair 1.8 µm Shim-pack Velox UHPLC 
columns with an Shim-pack Velox UHPLC Precolumn filter instead of a guard cartridge.

Shim-pack Velox UHPLC Precolumn Filter (0.2 µm)

ID(mm)

Length(mm)

3

2

Maximize LC Separation Performance
with Core Shell Technology

Designed to maximize performance of LC systems, Shimadzu’s Shim-pack 
Velox columns with core shell technology enable you to achieve increased 
separations and faster analysis times on any LC platform.
Whether developing a high efficiency LC separation method, transferring an 
existing method for increased throughput while maintaining resolution, or 
are trying to improve the resolution of a complex separation, Shim-pack 
Velox columns will satisfy your needs.
Column ruggedness is  critical to any LC analysis and Shim-pack Velox 
core-shell columns deliver excellent column lifetime for even the most 
challenging sample matrices.

Increased resolution with maximum efficiency 

      improving separation and detection

Faster separation without sacrificing performance 

      maximizing laboratory productivity and reducing cost of analysis

Increased sample throughput 

      reducing overall analysis time

Superior ruggedness 

      reducing cost of analysis 

Excellent reproducibility 

      maintaining analysis and data integrity

Prominence

Prominence-i plus

Nexera XR

Nexera-i plus

Nexera X2

* For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 80MPa.

Shim-pack Velox column will deliver

Combining highly efficient core shell particle technology with a wide range of surface chemistries provides you with the best 
opportunity for optimal resolution. With different chemistry characteristics, Shim-pack Velox columns are suitable for use in a wide 
variety of applications and challenging separations.

Column Chemistries

We understand that lot to lot consistent performance of columns is required to maximize your laboratory performance. From one lot 
to the next, every Shim-pack Velox column you purchase will perform consistently. 

Lot to Lot Reproducibility

Column particle sizes and column volumes affect chromatography results significantly if the column configuration does not match 
the LC system. As column particle size is reduced, or the column volume (ID and/or the length of the columns) decreases, the 
necessity for a lower dispersion system is increased.
Choosing the optimal column configuration for your LC system allows you to achieve improved chromatography.  The following 
table summarizes the starting recommendations of column configuration for each LC system.

Column Selection Guide for Different LC Platforms

LC System

Particle size
Column I.D.

Column Length

USP Classification
Ligand type
Particle Size (µm)
Pore size (

Å)

SP-C18

L1

Sterically protected C18

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

7 %
7 %
5 %

No

1.0-8.0

100 MPa*

60 MPa
40 MPa

C18

L1

C18

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

9 %
7 %
5 %

YES

2.0-8.0

100 MPa*

60 MPa
40 MPa

Biphenyl

L11

Biphenyl

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

7 %
7 %
5 %

YES

1.5-8.0

100 MPa*

60 MPa
40 MPa

Compounds (50 ng/mL) :
1. Cortisol
2. 11-Deoxycortisol
3. Estradiol
4. Boldenone
5. Testosterone
6. Androstenedione
7. Progesterone

Lot 1

Lot 2

Lot 3

Lot 4

0.0

0.50

1.00

1.50

2.00

Time (min)

2.50

3.00

3.50

4.00

Lot 5

Column: Shim-pack Velox Biphenyl 2.7 µm, 3.0×100 mm
                (P/N: 227-32016-03 )
Flow Rate: 0.7 mL/min
Column Temp.: 30 °C
Sample Dilulent: Initial mobile phase 
Injection Volume: 5 µL
Mobile Phase A: 0.1 % formic acid  in water
Mobile Phase B: 0.1 % formic acid in acetonitrile
Gradient: 40 % B (0 min)      80 % B (3 min) 
                

      40 %B (3.01 min – 5 min)

PFPP

L43

Pentafluorophenyl propyl

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

4 %
4 %
3 %

No

2.0-8.0

100 MPa*

60 MPa
40 MPa

HILIC

L3

None

2.7

90

130 m2/g

N/A

No

2.0-8.0

60 MPa

End-Cap
pH range

Suraface Area

Carbon Load

1.8 µm
2.7 µm
5 µm
1.8 µm
2.7 µm
5 µm

1.8 µm
2.7 µm
5 µm

Max Pressure

HPLC

2.7 µm & 5 µm

4.6 mm (3.0 mm)

100-250 mm

UHPLC-like

2.7 µm

3.0 mm (2.1 mm)

50-100 mm

UHPLC

1.8 µm & 2.7 µm

2.1 mm

≤150 mm

Shim-pack Velox LC Columns

Maximize LC Separation Performance with Core Shell Technology

Si

O

Si

O

Si

O

Si

F

F

F

F

F

O

3

2

Maximize LC Separation Performance
with Core Shell Technology

Designed to maximize performance of LC systems, Shimadzu’s Shim-pack 
Velox columns with core shell technology enable you to achieve increased 
separations and faster analysis times on any LC platform.
Whether developing a high efficiency LC separation method, transferring an 
existing method for increased throughput while maintaining resolution, or 
are trying to improve the resolution of a complex separation, Shim-pack 
Velox columns will satisfy your needs.
Column ruggedness is  critical to any LC analysis and Shim-pack Velox 
core-shell columns deliver excellent column lifetime for even the most 
challenging sample matrices.

Increased resolution with maximum efficiency 

      improving separation and detection

Faster separation without sacrificing performance 

      maximizing laboratory productivity and reducing cost of analysis

Increased sample throughput 

      reducing overall analysis time

Superior ruggedness 

      reducing cost of analysis 

Excellent reproducibility 

      maintaining analysis and data integrity

Prominence

Prominence-i plus

Nexera XR

Nexera-i plus

Nexera X2

* For maximum lifetime, recommended maximum pressure for 1.8 µm particles is 80MPa.

Shim-pack Velox column will deliver

Combining highly efficient core shell particle technology with a wide range of surface chemistries provides you with the best 
opportunity for optimal resolution. With different chemistry characteristics, Shim-pack Velox columns are suitable for use in a wide 
variety of applications and challenging separations.

Column Chemistries

We understand that lot to lot consistent performance of columns is required to maximize your laboratory performance. From one lot 
to the next, every Shim-pack Velox column you purchase will perform consistently. 

Lot to Lot Reproducibility

Column particle sizes and column volumes affect chromatography results significantly if the column configuration does not match 
the LC system. As column particle size is reduced, or the column volume (ID and/or the length of the columns) decreases, the 
necessity for a lower dispersion system is increased.
Choosing the optimal column configuration for your LC system allows you to achieve improved chromatography.  The following 
table summarizes the starting recommendations of column configuration for each LC system.

Column Selection Guide for Different LC Platforms

LC System

Particle size
Column I.D.

Column Length

USP Classification
Ligand type
Particle Size (µm)
Pore size (

Å)

SP-C18

L1

Sterically protected C18

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

7 %
7 %
5 %

No

1.0-8.0

100 MPa*

60 MPa
40 MPa

C18

L1

C18

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

9 %
7 %
5 %

YES

2.0-8.0

100 MPa*

60 MPa
40 MPa

Biphenyl

L11

Biphenyl

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

7 %
7 %
5 %

YES

1.5-8.0

100 MPa*

60 MPa
40 MPa

Compounds (50 ng/mL) :
1. Cortisol
2. 11-Deoxycortisol
3. Estradiol
4. Boldenone
5. Testosterone
6. Androstenedione
7. Progesterone

Lot 1

Lot 2

Lot 3

Lot 4

0.0

0.50

1.00

1.50

2.00

Time (min)

2.50

3.00

3.50

4.00

Lot 5

Column: Shim-pack Velox Biphenyl 2.7 µm, 3.0×100 mm
                (P/N: 227-32016-03 )
Flow Rate: 0.7 mL/min
Column Temp.: 30 °C
Sample Dilulent: Initial mobile phase 
Injection Volume: 5 µL
Mobile Phase A: 0.1 % formic acid  in water
Mobile Phase B: 0.1 % formic acid in acetonitrile
Gradient: 40 % B (0 min)      80 % B (3 min) 
                

      40 %B (3.01 min – 5 min)

PFPP

L43

Pentafluorophenyl propyl

1.8, 2.7, 5

90

125 m2/g
130 m2/g
100 m2/g

4 %
4 %
3 %

No

2.0-8.0

100 MPa*

60 MPa
40 MPa

HILIC

L3

None

2.7

90

130 m2/g

N/A

No

2.0-8.0

60 MPa

End-Cap
pH range

Suraface Area

Carbon Load

1.8 µm
2.7 µm
5 µm
1.8 µm
2.7 µm
5 µm

1.8 µm
2.7 µm
5 µm

Max Pressure

HPLC

2.7 µm & 5 µm

4.6 mm (3.0 mm)

100-250 mm

UHPLC-like

2.7 µm

3.0 mm (2.1 mm)

50-100 mm

UHPLC

1.8 µm & 2.7 µm

2.1 mm

≤150 mm

Shim-pack Velox LC Columns

Maximize LC Separation Performance with Core Shell Technology

Si

O

Si

O

Si

O

Si

F

F

F

F

F

O

5

4

Shim-pack Velox SP-C18

Shim-pack Velox C18

Designed and intended specifically for use under low pH condition, Shim-pack Velox
SP (Sterically Protected)-C18 offers a well balanced retention profile with a long life 
time even under harsh, acidic condition needed for LC/MS(/MS) analysis.

Sterically protected to resist strongly acidic (pH 1-3) mobile phase condition
Well balanced retention profile
Suitable for LC/MS(/MS) analysis

Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the 
highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a 
wide range of applications such as pharmaceutical, food, environmental and clinical 
and neutrals at moderately low and mid-range pH.

General purpose column for reversed-phase chromatography
Highest hydrophobic retention among Shim-pack Velox series
Compatible with moderately acidic to neutral mobile phases (pH 2-8) 

Sterically protected ligand provides extended low pH stability for the core shell particle. Shim-pack Velox SP-C18 columns maintain a 
stable retention profile under strongly acidic mobile phase condition (pH1) .

The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 µm fully-porous ODS column described in the USP monograph 
is transferred to a new method with Shim-pack Velox C18 2.7 µm column, within USP allowable adjustments. Analytical time and 
solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.

USP requirement of Chromatography <621>
When the column size is changed, the following conditions should be met;
  1) L/dp ratio: within -25 % to +50 %
 or
      Number of theoretical plates (N): within -25 % to +50 % (For SPP)
  2) Flow rate: *Based on particle size and internal diameter and ±50%

Low pH Stability

More than 20 compounds used as the preservative agent for industrial products like foods and cosmetics can can be separated by 
Shim-pack Velox SP-C18. The simultaneous determination and quantitation of multiple target compounds are possible in a wide 
range of commercial product within acceptable analytical times.

Simultaneous Analysis of 22 Preservative Agents

Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment

Flow Rate : 0.6 mL/min
Column Temp. : 60 °C
Mobile Phase A : 0.1 % TFA in water
Mobile Phase B : Acetonitrile
Gradient : 45 % B (0 min – 7.5 min)      5 % B (7.51 min – 50 min) 
 

    100 % B (55 min )      45 % B (55.01 min      60 min)

Preservative agents

Magnified Baseline

LC Conditions
Column: Shim-pack Velox SP-C18, 2.7 µm, 3.0×100 mm (PN: 227-32004-03)
Flow Rate: 1.0 mL/min
Column Temp.: 45

 °C

Mobile Phase A: NaH2PO4 aq. (25 mM, pH3.8)
Mobile Phase B: MeOH/ACN = 9/1
Gradient: 8 %B (0 min ) 

    30 % B (0.7 min – 2.7 min)     47 % B (2.71 min – 5min )     52 % 

B (6 min) 

    80 %B (7 min – 8 min)      8 %B (9.01 min -10 min)

Injection Volume: 1 µL
Conc.: 50 mg/L

1 2-methyl-4-Isothiazolin-3-one
2 2-Bromo-2-Nitro-1,3-propanediol
3 Salicylic Acid
4 Isothiazolinones
5 Benzoic Acid
6 Benzyl Alcohol
7 Sorbic Acid
8 2-Phenoxyethanol
9 Methyl Paraben
10 Ethyl Paraben
11 Methyl Benzoate

12 Isopropyl Paraben
13 Propyl Paraben
14 4-Chloro-3-Methylphenol
15 Ethyl Benzoate
16 Isobutyl 4-Hydroxybenzoate
17 Butyl Paraben
18 Chloroxylenol
19 Phenyl Benzoate
20 Clorofene
21 Triclocarban
22 Triclosan

Original Method
Shim-pack VP-ODS (5 µm, 4.6×150 mm)
Flow rate: 0.5 mL/min
Injection Volume: 100 µL
Column temperature: 25 °C
Detection: UV 361 nm

Column

VP-ODS

(5 µm, 4.6×150 mm)

Velox C18

(2.7 µm, 4.6×100 mm)

L/dp

30,000

37,037

(+23 %)

Flow rate
(mL/min)

0.50

0.50

0.93

5,244

9,497

(+81 %)

4,466

(-15 %)

tR: 0.025 %

Area: 0.175 %  (n=6)

tR: 0.035 %

Area: 0.103 % (n=6)

tR: 0.084 %

Area: 0.220 % (n=6)

N

System suitability test result
(Requirement: %RSD < 2.0 %)

Faster Method
Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate: 0.50 mL/min
Injection Volume: 67 µL
Column temperature: 25 °C
Detection: UV 361 nm

Optimized Method
Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate: 0.93 mL/min
Injection Volume: 67 µL
Column temperature: 25 °C
Detection: UV 361 nm

% loss of retention (k) vs. hours at pH 1

5

0

-5

-10

-15

-20

-25

% loss k

Hours

5.163 min

2.368min

1.297 min

0

10

20

30

40

50

60

70

80

90

100

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

min

Shim-pack Velox SP-C18
Brand A FPP C18
Brand B FPP C18
Brand C FPP C18
Brand D Core-shell C18
Brand E Core-shell C18
Brand F Core-shell C18

Shim-pack Velox LC Columns

Maximize LC Separation Performance with Core Shell Technology

Si

O

Si

O

L    

：Column length 

dp 

：Particle size

F    

：Flow rate

dc  

：Internal diameter of the column

* F2 = F1 ×

dc22 × dp1
dc12 × dp2

254 nm, 280 nm

254 nm, 280 nm

0

40

80

120

mAU

0.0

2.0

4.0

6.0

8.0

min

0

20

40

60

1

2

3 4 6 5 8

7

9

10

1114

12 13

15 18

16 17 19

20

21

22

1

3 4

5 8

7

9

10

1114

1213

15 18

1617 19

20

21

22

1

2

3

4

5 8

7

9

10

1114

1213

15 18

16 17 19

20

21

22

0.0

10.0

20.0

30.0

0.0

2.0

4.0

6.0

8.0

min

1

2

3 4 6

58

7

9

10

11

14

12 13

15

18

16

17

19

20

21

22

0.0

10.0

20.0

30.0

mAU

5

4

Shim-pack Velox SP-C18

Shim-pack Velox C18

Designed and intended specifically for use under low pH condition, Shim-pack Velox
SP (Sterically Protected)-C18 offers a well balanced retention profile with a long life 
time even under harsh, acidic condition needed for LC/MS(/MS) analysis.

Sterically protected to resist strongly acidic (pH 1-3) mobile phase condition
Well balanced retention profile
Suitable for LC/MS(/MS) analysis

Shim-pack Velox C18 is a traditional end-capped C18-bonded phase which offers the 
highest hydrophobic retention of any Shim-pack Velox phases and is applicable to a 
wide range of applications such as pharmaceutical, food, environmental and clinical 
and neutrals at moderately low and mid-range pH.

General purpose column for reversed-phase chromatography
Highest hydrophobic retention among Shim-pack Velox series
Compatible with moderately acidic to neutral mobile phases (pH 2-8) 

Sterically protected ligand provides extended low pH stability for the core shell particle. Shim-pack Velox SP-C18 columns maintain a 
stable retention profile under strongly acidic mobile phase condition (pH1) .

The assay of cyanocobalamin (a synthetic form of vitamin B12) with 5 µm fully-porous ODS column described in the USP monograph 
is transferred to a new method with Shim-pack Velox C18 2.7 µm column, within USP allowable adjustments. Analytical time and 
solvent consumption can be saved with transferred methods while meeting the requirements of system suitability.

USP requirement of Chromatography <621>
When the column size is changed, the following conditions should be met;
  1) L/dp ratio: within -25 % to +50 %
 or
      Number of theoretical plates (N): within -25 % to +50 % (For SPP)
  2) Flow rate: *Based on particle size and internal diameter and ±50%

Low pH Stability

More than 20 compounds used as the preservative agent for industrial products like foods and cosmetics can can be separated by 
Shim-pack Velox SP-C18. The simultaneous determination and quantitation of multiple target compounds are possible in a wide 
range of commercial product within acceptable analytical times.

Simultaneous Analysis of 22 Preservative Agents

Method Transfer for Cyanocobalamin Analysis within the USP Allowable Adjustment

Flow Rate : 0.6 mL/min
Column Temp. : 60 °C
Mobile Phase A : 0.1 % TFA in water
Mobile Phase B : Acetonitrile
Gradient : 45 % B (0 min – 7.5 min)      5 % B (7.51 min – 50 min) 
 

    100 % B (55 min )      45 % B (55.01 min      60 min)

Preservative agents

Magnified Baseline

LC Conditions
Column: Shim-pack Velox SP-C18, 2.7 µm, 3.0×100 mm (PN: 227-32004-03)
Flow Rate: 1.0 mL/min
Column Temp.: 45

 °C

Mobile Phase A: NaH2PO4 aq. (25 mM, pH3.8)
Mobile Phase B: MeOH/ACN = 9/1
Gradient: 8 %B (0 min ) 

    30 % B (0.7 min – 2.7 min)     47 % B (2.71 min – 5min )     52 % 

B (6 min) 

    80 %B (7 min – 8 min)      8 %B (9.01 min -10 min)

Injection Volume: 1 µL
Conc.: 50 mg/L

1 2-methyl-4-Isothiazolin-3-one
2 2-Bromo-2-Nitro-1,3-propanediol
3 Salicylic Acid
4 Isothiazolinones
5 Benzoic Acid
6 Benzyl Alcohol
7 Sorbic Acid
8 2-Phenoxyethanol
9 Methyl Paraben
10 Ethyl Paraben
11 Methyl Benzoate

12 Isopropyl Paraben
13 Propyl Paraben
14 4-Chloro-3-Methylphenol
15 Ethyl Benzoate
16 Isobutyl 4-Hydroxybenzoate
17 Butyl Paraben
18 Chloroxylenol
19 Phenyl Benzoate
20 Clorofene
21 Triclocarban
22 Triclosan

Original Method
Shim-pack VP-ODS (5 µm, 4.6×150 mm)
Flow rate: 0.5 mL/min
Injection Volume: 100 µL
Column temperature: 25 °C
Detection: UV 361 nm

Column

VP-ODS

(5 µm, 4.6×150 mm)

Velox C18

(2.7 µm, 4.6×100 mm)

L/dp

30,000

37,037

(+23 %)

Flow rate
(mL/min)

0.50

0.50

0.93

5,244

9,497

(+81 %)

4,466

(-15 %)

tR: 0.025 %

Area: 0.175 %  (n=6)

tR: 0.035 %

Area: 0.103 % (n=6)

tR: 0.084 %

Area: 0.220 % (n=6)

N

System suitability test result
(Requirement: %RSD < 2.0 %)

Faster Method
Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate: 0.50 mL/min
Injection Volume: 67 µL
Column temperature: 25 °C
Detection: UV 361 nm

Optimized Method
Shim-pack Velox C18 (2.7 µm, 4.6×100 mm)
Flow rate: 0.93 mL/min
Injection Volume: 67 µL
Column temperature: 25 °C
Detection: UV 361 nm

% loss of retention (k) vs. hours at pH 1

5

0

-5

-10

-15

-20

-25

% loss k

Hours

5.163 min

2.368min

1.297 min

0

10

20

30

40

50

60

70

80

90

100

0.0

1.0

2.0

3.0

4.0

5.0

6.0

7.0

8.0

9.0

min

Shim-pack Velox SP-C18
Brand A FPP C18
Brand B FPP C18
Brand C FPP C18
Brand D Core-shell C18
Brand E Core-shell C18
Brand F Core-shell C18

Shim-pack Velox LC Columns

Maximize LC Separation Performance with Core Shell Technology

Si

O

Si

O

L    

：Column length 

dp 

：Particle size

F    

：Flow rate

dc  

：Internal diameter of the column

* F2 = F1 ×

dc22 × dp1
dc12 × dp2

254 nm, 280 nm

254 nm, 280 nm

0

40

80

120

mAU

0.0

2.0

4.0

6.0

8.0

min

0

20

40

60

1

2

3 4 6 5 8

7

9

10

1114

12 13

15 18

16 17 19

20

21

22

1

3 4

5 8

7

9

10

1114

1213

15 18

1617 19

20

21

22

1

2

3

4

5 8

7

9

10

1114

1213

15 18

16 17 19

20

21

22

0.0

10.0

20.0

30.0

0.0

2.0

4.0

6.0

8.0

min

1

2

3 4 6

58

7

9

10

11

14

12 13

15

18

16

17

19

20

21

22

0.0

10.0

20.0

30.0

mAU

7

6

Shim-pack Velox Biphenyl

Shim-pack Velox PFPP

Shim-pack Velox HILIC

Shim-pack Velox Biphenyl provides enhanced retention of aromatic compounds. 
It is useful for fast separations in bioanalytical applications due to the increased 
retention of early eluting analytes such as dipolar, unsaturated and conjugated 
analytes.

Complementary selectivity to alkyl phases
Enhanced separation of aromatic compounds
Ideal for increasing sensitivity and selectivity in LC/MS(/MS) analysis 

Amobarbital
5-ethyl-5-(3-methylbutyl)-
1,3-diazinane-2,4,6-trione

(× 1,000,000)

uV

1. cis-Stilbene

2. trans-Stilbene

1

2

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 min

1.0

1.5

2.0

0.0

1.5

1

2

3

4

5

O

O

O

NH

NH

NH2

NH2

NH2

NH

O

O

O

O

O

O

O

HO

HO

HO

HO

OH

OH OH

OH OH

OH OH

OH

N

H

N

N

N

N

N

N

N

N

N

N

1. Toluene

2. Uracil

3. Uridine 4. Adenosine

5. Guanosine

6. Cytidine

6

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

7.0

7.5

8.0

8.5

9.0

9.5 min

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

min

2.00

1.75

1.50

1.25

1.00

0.75

0.50

0.25

0.00

200000

150000

100000

50000

400000

300000

200000

100000

Chromatogram of 56 compounds in human urine spiked at the cut off concentration

Pentobarbital
5-ethyl-5-pentan-2-yl-
1,3-diazinane-2,4,6-trione

System: Nexera UHPLC System / LCMS-8050
Column: Shim-pack Velox Biphenyl 2.7 µ

ｍ, 2.1 mm × 100 mm

                (PN: 227-32015-03)
Column Temp.: 30 °C
Flow Rate: 0.5 mL/min
Mobile Phase A: 0.15 mM ammonium fluoride in water
Mobile Phase B: Methanol
Gradient: 20 % B (0 min)     48 % B (1.5 min)     53 % B (4 min)      
     100 %B (6 min - 7.5 min)     20 % B (7.51 min - 9.5 min)
Sample preparation:
Enzymatically hydrolyzed human urine was spiked with target 
compounds between 10 - 1000 % of the required cut off concentration. 
Samples were subsequently diluted 5x with 0.1 % formic acid.

LC Conditions
Column: Shim-pack Velox HILIC, 2.7 µm, 3.0×100 mm (PN: 227-32026-02)
Column Temp.: 30

℃

Flow Rate : 0.4 mL/min
Mobile Phase : Acetonitrile/20mM AcONH4aq.=9/1
Detection : UV 254 nm
Sample1. Toluene, 2. Uracil, 3. Uridine,  4. Adenosine, 5. Guanosine, 6. Cytidine
Injection Volume : 1 µL

LC Conditions
Column: Shim-pack Velox PFPP, 2.7 µm, 3.0×100 mm (PN: 227-32022-04)
Column Temp.: 40 °C
Flow Rate  : 0.4 mL/min
Mobile Phase  : Methanol/Water = 9/1
Detection : UV 254 nm
Sample: 1. cis-Stilbene,  2. trans-Stilbene
Injection Volume : 1 µL

Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation 
mode that can be used to improve the retention of challenging polar analytes. 
Shim-pack Velox HILIC using unbonded core shell particles is specifically designed for  
this application.

Orthogonal selectivity to reversed phase chromatography
Increased retention of polar analytes
Increased MS sensitivity 
Direct compatibility with sample preparation eluates

Ordering Information

Shim-pack Velox 1.8 µm Columns

Shim-pack Velox 2.7 µm Columns

Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to 
C18 columns and is suitable for the analysis of halogenated compounds, positional 
isomers and charged bases.

Alternative selectivity to C18 columns
Suitable for positional isomers and halogenated compounds
Offers increased retention for charged bases

Even under the condition where 56 drugs of abuse and metabolites in human urine are quantitated within 10 minutes, two 
structural isomers, amobarmital and pentobarbital, which have been historically difficult to separate due to their similarity in 
chemical structures, could be relatively well resolved with shim-pack Velox Biphenyl column.

Separating the Structural Isomers

Nucleosides are polar molecules that are not well retained on reversed phase LC columns due to their hydrophilic nature are well 
retained and separated with Shim-pack Velox HILIC column.

Retention of Nucleosides

Cis and trans isomers of stilbene that are difficult to resolve with an ODS column due to their similarity in hydrophobicity can be well 
separated with Shim-pack Velox PFPP column.

Good Separation of Cis / Trans Stilbene

Chemistry

30
50

100
150

SP-C18

2.1

227-32001-01
227-32001-02
227-32001-03
227-32001-04

3.0

–

227-32002-01
227-32002-02

–

C18

2.1

227-32007-01
227-32007-02
227-32007-03
227-32007-04

3.0

–

227-32008-01
227-32008-02

–

Biphenyl

2.1

227-32013-01
227-32013-02
227-32013-03
227-32013-04

3.0

–

227-32014-01
227-32014-02

–

PFPP

2.1

227-32019-01
227-32019-02
227-32019-03
227-32019-04

3.0

–

227-32020-01
227-32020-02

–

Chemistry

30
50

100
150

SP-C18

2.1

227-32003-01
227-32003-02
227-32003-03
227-32003-04

3.0 

227-32004-01
227-32004-02
227-32004-03
227-32004-04

4.6

227-32005-01
227-32005-02
227-32005-03
227-32005-04

C18

2.1 

227-32009-01
227-32009-02
227-32009-03
227-32009-04

3.0

227-32010-01
227-32010-02
227-32010-03
227-32010-04

Biphenyl

4.6 

227-32011-01
227-32011-02
227-32011-03
227-32011-04

2.1

227-32015-01
227-32015-02
227-32015-03
227-32015-04

3.0 

227-32016-01
227-32016-02
227-32016-03
227-32016-04

4.6

227-32017-01
227-32017-02
227-32017-03
227-32017-04

Chemistry

30
50

100
150

PFPP

2.1

227-32021-01
227-32021-02
227-32021-03
227-32021-04

3.0 

227-32022-01
227-32022-02
227-32022-03
227-32022-04

4.6

227-32023-01
227-32023-02
227-32023-03
227-32023-04

HILIC

2.1 

227-32025-01
227-32025-02
227-32025-03
227-32025-04

3.0

–

227-32026-01
227-32026-02
227-32026-03

4.6 

–

227-32027-01
227-32027-02
227-32027-03

Shim-pack Velox LC Columns

Maximize LC Separation Performance with Core Shell Technology

(× 100,000)

1.75

1.50

1.25

1.00

0.75

0.50

0.25

0.00

4.3  4.5  4.7  4.9 

Amobarbital

Pentobarbital

Si

O

Si

F

F

F

F

F

O

ID(mm)

Length(mm)

ID(mm)

Length(mm)

ID(mm)

Length(mm)

7

6

Shim-pack Velox Biphenyl

Shim-pack Velox PFPP

Shim-pack Velox HILIC

Shim-pack Velox Biphenyl provides enhanced retention of aromatic compounds. 
It is useful for fast separations in bioanalytical applications due to the increased 
retention of early eluting analytes such as dipolar, unsaturated and conjugated 
analytes.

Complementary selectivity to alkyl phases
Enhanced separation of aromatic compounds
Ideal for increasing sensitivity and selectivity in LC/MS(/MS) analysis 

Amobarbital
5-ethyl-5-(3-methylbutyl)-
1,3-diazinane-2,4,6-trione

(× 1,000,000)

uV

1. cis-Stilbene

2. trans-Stilbene

1

2

0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5 6.0 6.5 7.0 7.5 8.0 8.5 9.0 9.5 min

1.0

1.5

2.0

0.0

1.5

1

2

3

4

5

O

O

O

NH

NH

NH2

NH2

NH2

NH

O

O

O

O

O

O

O

HO

HO

HO

HO

OH

OH OH

OH OH

OH OH

OH

N

H

N

N

N

N

N

N

N

N

N

N

1. Toluene

2. Uracil

3. Uridine 4. Adenosine

5. Guanosine

6. Cytidine

6

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

7.0

7.5

8.0

8.5

9.0

9.5 min

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

6.5

min

2.00

1.75

1.50

1.25

1.00

0.75

0.50

0.25

0.00

200000

150000

100000

50000

400000

300000

200000

100000

Chromatogram of 56 compounds in human urine spiked at the cut off concentration

Pentobarbital
5-ethyl-5-pentan-2-yl-
1,3-diazinane-2,4,6-trione

System: Nexera UHPLC System / LCMS-8050
Column: Shim-pack Velox Biphenyl 2.7 µ

ｍ, 2.1 mm × 100 mm

                (PN: 227-32015-03)
Column Temp.: 30 °C
Flow Rate: 0.5 mL/min
Mobile Phase A: 0.15 mM ammonium fluoride in water
Mobile Phase B: Methanol
Gradient: 20 % B (0 min)     48 % B (1.5 min)     53 % B (4 min)      
     100 %B (6 min - 7.5 min)     20 % B (7.51 min - 9.5 min)
Sample preparation:
Enzymatically hydrolyzed human urine was spiked with target 
compounds between 10 - 1000 % of the required cut off concentration. 
Samples were subsequently diluted 5x with 0.1 % formic acid.

LC Conditions
Column: Shim-pack Velox HILIC, 2.7 µm, 3.0×100 mm (PN: 227-32026-02)
Column Temp.: 30

℃

Flow Rate : 0.4 mL/min
Mobile Phase : Acetonitrile/20mM AcONH4aq.=9/1
Detection : UV 254 nm
Sample1. Toluene, 2. Uracil, 3. Uridine,  4. Adenosine, 5. Guanosine, 6. Cytidine
Injection Volume : 1 µL

LC Conditions
Column: Shim-pack Velox PFPP, 2.7 µm, 3.0×100 mm (PN: 227-32022-04)
Column Temp.: 40 °C
Flow Rate  : 0.4 mL/min
Mobile Phase  : Methanol/Water = 9/1
Detection : UV 254 nm
Sample: 1. cis-Stilbene,  2. trans-Stilbene
Injection Volume : 1 µL

Hydrophilic interaction chromatography (HILIC) is an increasingly popular separation 
mode that can be used to improve the retention of challenging polar analytes. 
Shim-pack Velox HILIC using unbonded core shell particles is specifically designed for  
this application.

Orthogonal selectivity to reversed phase chromatography
Increased retention of polar analytes
Increased MS sensitivity 
Direct compatibility with sample preparation eluates

Ordering Information

Shim-pack Velox 1.8 µm Columns

Shim-pack Velox 2.7 µm Columns

Shim-pack Velox PFPP (Pentafluorophenylpropyl) provides an alternative selectivity to 
C18 columns and is suitable for the analysis of halogenated compounds, positional 
isomers and charged bases.

Alternative selectivity to C18 columns
Suitable for positional isomers and halogenated compounds
Offers increased retention for charged bases

Even under the condition where 56 drugs of abuse and metabolites in human urine are quantitated within 10 minutes, two 
structural isomers, amobarmital and pentobarbital, which have been historically difficult to separate due to their similarity in 
chemical structures, could be relatively well resolved with shim-pack Velox Biphenyl column.

Separating the Structural Isomers

Nucleosides are polar molecules that are not well retained on reversed phase LC columns due to their hydrophilic nature are well 
retained and separated with Shim-pack Velox HILIC column.

Retention of Nucleosides

Cis and trans isomers of stilbene that are difficult to resolve with an ODS column due to their similarity in hydrophobicity can be well 
separated with Shim-pack Velox PFPP column.

Good Separation of Cis / Trans Stilbene

Chemistry

30
50

100
150

SP-C18

2.1

227-32001-01
227-32001-02
227-32001-03
227-32001-04

3.0

–

227-32002-01
227-32002-02

–

C18

2.1

227-32007-01
227-32007-02
227-32007-03
227-32007-04

3.0

–

227-32008-01
227-32008-02

–

Biphenyl

2.1

227-32013-01
227-32013-02
227-32013-03
227-32013-04

3.0

–

227-32014-01
227-32014-02

–

PFPP

2.1

227-32019-01
227-32019-02
227-32019-03
227-32019-04

3.0

–

227-32020-01
227-32020-02

–

Chemistry

30
50

100
150

SP-C18

2.1

227-32003-01
227-32003-02
227-32003-03
227-32003-04

3.0 

227-32004-01
227-32004-02
227-32004-03
227-32004-04

4.6

227-32005-01
227-32005-02
227-32005-03
227-32005-04

C18

2.1 

227-32009-01
227-32009-02
227-32009-03
227-32009-04

3.0

227-32010-01
227-32010-02
227-32010-03
227-32010-04

Biphenyl

4.6 

227-32011-01
227-32011-02
227-32011-03
227-32011-04

2.1

227-32015-01
227-32015-02
227-32015-03
227-32015-04

3.0 

227-32016-01
227-32016-02
227-32016-03
227-32016-04

4.6

227-32017-01
227-32017-02
227-32017-03
227-32017-04

Chemistry

30
50

100
150

PFPP

2.1

227-32021-01
227-32021-02
227-32021-03
227-32021-04

3.0 

227-32022-01
227-32022-02
227-32022-03
227-32022-04

4.6

227-32023-01
227-32023-02
227-32023-03
227-32023-04

HILIC

2.1 

227-32025-01
227-32025-02
227-32025-03
227-32025-04

3.0

–

227-32026-01
227-32026-02
227-32026-03

4.6 

–

227-32027-01
227-32027-02
227-32027-03

Shim-pack Velox LC Columns

Maximize LC Separation Performance with Core Shell Technology

(× 100,000)

1.75

1.50

1.25

1.00

0.75

0.50

0.25

0.00

4.3  4.5  4.7  4.9 

Amobarbital

Pentobarbital

Si

O

Si

F

F

F

F

F

O

ID(mm)

Length(mm)

ID(mm)

Length(mm)

ID(mm)

Length(mm)

Shim-pack V

elox LC Columns

C190-E219

Maximize LC Separation Performance with Core Shell Technology 

Shim-pack Velox LC Columns

© Shimadzu Corporation, 2018

www.shimadzu.com/an/

For Research Use Only. Not for use in diagnostic procedures. 

This publication may contain references to products that are not available in your country. Please contact us to check the availability of these 

products in your country.

Company names, products/service names and logos used in this publication are trademarks and trade names of Shimadzu Corporation, its 

subsidiaries or its affiliates, whether or not they are used with trademark symbol “TM” or “®”.

Third-party trademarks and trade names may be used in this publication to refer to either the entities or their products/services, whether or not 

they are used with trademark symbol “TM” or “®”.

Shimadzu disclaims any proprietary interest in trademarks and trade names other than its own.

The contents of this publication are provided to you “as is” without warranty of any kind, and are subject to change without notice. Shimadzu 

does not assume any responsibility or liability for any damage, whether direct or indirect, relating to the use of this publication.

First Edition: September 2018, Printed in Japan 3655-08801-20AIK

Free-turning architecture lets you change cartridges by hand without breaking inlet / outlet fluid connections — no tools needed.
Guard column cartridges require Shim-pack Velox EXP Direct Connect Holder (227-32041-01)

Shim-pack Velox EXP Guard Column

Tubing

Nut

Ferrule

Peek End

Ferrule

Peek End

Holder Cap

Holder Body

Guard Column

Cartridge

Ordering Information

Shim-pack Velox 5 µm Columns

Shim-pack Velox EXP Guard Column Cartridge (3 pk)

Shim-pack Velox UHPLC Precolumn Filter (0.2 µ

ｍ)

＊Shim-pack Velox EXP Direct Connect Holder : 227-32041-01

Chemistry

50

100
150
250

SP-C18

227-32006-01
227-32006-02
227-32006-03
227-32006-04

C18

227-32012-01
227-32012-02
227-32012-03
227-32012-04

Biphenyl

227-32018-01
227-32018-02
227-32018-03
227-32018-04

4.6 

PFPP

227-32024-01
227-32024-02
227-32024-03
227-32024-04

Type

ID (mm)

2.1
3.0
4.6

UHPLC

SP-C18

227-32028-01
227-32028-02

–

C18

227-32031-01
227-32031-02

–

Biphenyl

227-32034-01
227-32034-02

–

PFPP

227-32037-01
227-32037-02

–

SP-C18

227-32029-01
227-32029-02
227-32029-03

2.7 µ

ｍ

C18

227-32032-01
227-32032-02
227-32032-03

Biphenyl

227-32035-01
227-32035-02
227-32035-03

PFPP

227-32038-01
227-32038-02
227-32038-03

HILIC

227-32040-01
227-32040-02
227-32040-03

Type

ID (mm)

4.6

5 µ

ｍ

SP-C18

227-32030-01

C18

227-32033-01

Biphenyl

227-32036-01

PFPP

227-32039-01

Part No. 1 pack
Part No. 5 pack
Part No. 10 pack

227-32042-01
227-32042-02
227-32042-03

To minimize extra column volume and maximize UHPLC sample throughput with SPE, 
SLE, or other sample preparation techniques, pair 1.8 µm Shim-pack Velox UHPLC 
columns with an Shim-pack Velox UHPLC Precolumn filter instead of a guard cartridge.

Shim-pack Velox UHPLC Precolumn Filter (0.2 µm)

ID(mm)

Length(mm)