Characterizing Antibody-Drug Conjugates and Assigning Drug Conjugation Sites

Agilent BioHPLC columns Characterization of Antibody-Drug Conjugate Critical Quality Attributes Application compendium Contents Introduction3 Featured application notes 2 PLRP-S Polymeric Reversed-Phase Column for LC/MS Separation of mAbs and ADC (5991-7163EN) 5 Intact- and Peptide-Level Characterization of Trastuzumab Emtansine In Vitro…

Analysis of Cysteine-Linked Antibody Drug Conjugates Using Hydrophobic Interaction Chromatography on the Agilent 1260 Infinity II Bio-inert LC Application Note Biologics and Biosimilars Author Abstract Sonja Schneider Hydrophobic interaction chromatography (HIC) is frequently used for the Agilent Technologies, Inc. determination…

Application Note Biopharma/Pharma Characterization of Antibody‑Drug Conjugates Using 2D-LC and Native MS Authors David L. Wong and Sarah M. Stow Agilent Technologies, Inc. Abstract Antibody drug conjugates (ADCs), which comprise a monoclonal antibody (mAb) conjugated to a small molecule drug…

Mapping the Drug Conjugation Sites of an Antibody-Drug Conjugate Using Automated Sample Preparation and LC/MS Analysis Application Note Authors Introduction Jing Chen Antibody drug conjugates (ADCs) that combine the specificity of monoclonal antibodies (mAbs) with the potency of cytotoxic molecules…