Size-Exclusion Chromatography Analysis of Adeno-Associated Virus (AAV) Preparations Using a 450 Å Diol-Bonded BEH Column and Fluorescence Detection

[ APPLICATION NOTE ] Anion-Exchange Chromatography for Determining Empty and Full Capsid Contents in Adeno-Associated Virus Hua Yang, Stephan Koza, and Weibin Chen Waters Corporation, Milford, MA, USA APPLICATION BENEFITS ■ ■ Empty and full AAV capsid separation Recombinant adeno-associated…

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

Developing the analytics and analytical workflows supporting the analysis of the next generation of biotherapeutic and gene therapies. Scott J. Berger, Ph.D. and Ximo Zhang, Ph.D. Waters Lunch Seminar Tuesday January 28, 2020 WCBP 2020 Diamond Program Partner ©2020 Waters…

Application Note Improved SizeExclusion Chromatography of Adeno-Associated Viruses (AAVs) With XBridge™ Premier GTx BEH™ SEC 450 Å 2.5 µm Columns Mateusz Imiolek, Szabolcs Fekete, Matthew A. Lauber Waters Corporation Abstract This application note discusses the use of XBridge™ Premier GTx…