Back to Basics - Gradient Retention Factor, K*

Back to Basics Gradient Anatomy Protocols for LC Instruments 2020 Why is Gradient Chromatography Needed? Analytical samples are quite frequently made of complex analytes with varying physico-chemical properties, such as polarity. The analysis of these compounds using isocratic conditions could…

Back to Basics Dispersion Protocols for LC Instruments 2020 What is Dispersion? Dispersion is a measure of analyte band broadening between the injector and detector, excluding the contribution from the column. As a sample is injected into the flow path,…

Back to Basics Explaining Resolution 2020 What is Resolution? Chromatography is the act of separating components in a mixture. There are different measurements to determine the success of a separation, one of which is resolution (Rs). Resolution is determined between…

Back to Basics Pump Linearity and Dwell Volume Measurements Protocols for LC Instruments 2020 How is the Linearity of the Pump Measured? The linearity of the pump should be assessed between 0-100% using the conditions described in Figure 1. This…