Vanquish HPLC and
UHPLC Systems
LC that takes
your productivity
to new heights
2
Today’s scientists need to solve the challenges faced to produce life-saving medicines
and improve the quality of our food and the environment. This work deserves the
best analytical tools available; tools that empower you to achieve your next scientific
breakthrough, measure the previously unmeasurable, and deliver outstanding quality
results dependably.
Designed with innovative technology and attention to fine detail, Thermo Scientific™
Vanquish™ HPLC and UHPLC systems are the most advanced LC instruments
available. The Vanquish systems improve performance and repeatability with no
trade-offs in quality, robustness, or ease-of-use. Regardless of which Vanquish HPLC
or UHPLC system is used, chromatographers have all they need to solve their
toughest analytical challenges with confidence.
• Deliver results without compromise—Unsurpassed retention time
and peak area precision
• Easier method development and routine analysis—Dedicated tools
for fast method development and validation with exceptional instrument
robustness to maximize uptime
• Improve productivity—Better throughput, improved sample
characterization, and faster return on investment
• Detect the lowest sample quantities—Higher detector sensitivity
and lower baseline noise
• Seamlessly integrate MS—Dedicated kits and software solutions
provide exceptional LC-MS performance
• Maximize your results—Easy to use, control, and process data with
Thermo Scientific™ Chromeleon™ Chromatography Data System (CDS)
Thermo Scientific™ Vanquish™
Core HPLC system
Absolute dependability to enable
worry-free applications
No trade-offs in performance, robustness or ease-of-use
Get more from liquid chromatography
Thermo Scientific™ Vanquish™
Horizon UHPLC system
Unrivaled performance and
throughput for applications
requiring high-end UHPLC
For any application—whether HPLC or UHPLC, small or
large molecules, simple or complex mixtures—the Vanquish
platform was designed to handle it all. This innovative,
simple-to-operate, and easy-to-maintain platform delivers
confident separations that push chromatographic boundaries.
Regardless of the configuration you choose, you will get a
highly integrated solution with optimized fluidic connections,
providing unsurpassed retention time and peak area precision.
Thermo Scientific™ Vanquish™
Flex UHPLC systems
Complete flexibility for method
development or fast and reliable
UHPLC
Thermo Scientific™ Vanquish™
Duo HPLC and UHPLC systems
Increased productivity with two
completely independent flow paths
for higher throughput and improved
sample characterization
Run more samples, unattended
3
Vanquish Horizon UHPLC system
The Thermo Scientific Vanquish Horizon UHPLC system is an
integrated, fully biocompatible state-of-the-art binary UHPLC system
with ultra-low gradient delay volume, designed to provide unrivaled
performance for high resolution and high-throughput LC and LC-MS
applications.
Why SmartInject Technology?
Unlike traditional UHPLC systems, Vanquish
LC systems utilize SmartInject Technology.
A novel injection feature that significantly
reduces dramatic pressure pulses that would
otherwise lead to reduced column lifetime
and challenges with retention time precision.
SmartInject Technology enables improved
data confidence and reduced cost of
ownership.
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Ultra high peak capacity for a complex herbal extract analysis.
0
4.0
6.0
5.0
10.0
15.0
20.0
25.0
30.0
Peak capacity of 720 at 1360 bar
Retention time [min]
Absorbance [mAU]
0
10
20
30
40
50
60
70
80
90
100
0
5.0
10.0
15.0
20.0
25.0
30.0
Impurity profiling of nevirapine (0.66 mg/mL) with UHPLC
gradient and zoom into the baseline to show related impurities.
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mAU
1
2
3
4
5
6
8
A
bs
o
rb
anc
e [mAU]
0.
1
3
.2
-2
0
Minutes
-500
0
0.0
0.5
1.0
1.2
Nevirapine
Minutes
3500
7
Significant reduction of pressure drop after injection using SmartInject
Technology resulting in improved retention time reproducibility (6 replicates)
and enhanced column lifetime.
System Pressure Trace
Time [min]
Inject
0
Without SmartInject
No pressure drop with SmartInject
Pressure drop
Pressure [bar]
UV Trace with SmartInject
Absorbance [mAU]
3
Best precision
Deliver results without compromise
Vanquish Flex UHPLC systems
The Thermo Scientific Vanquish Flex systems offer binary and
quaternary solvent blending. Binary solvent blending results in low
gradient delay volumes that are ideal for routine UHPLC and LC-MS
applications. Quaternary solvent blending allows multicomponent
gradient formation for flexible UHPLC method development.
Vanquish Core HPLC system
The Thermo Scientific Vanquish Core HPLC systems offers highly dependable
HPLC analysis for binary, quaternary and isocratic solvent blending. A new level of
user experience, the highest system productive time, and its ease to adopt within
any given laboratory infrastructure make it ideal for all traditional HPLC analyses.
High-throughput pesticides
analysis by full scan LC-MS
presented as extracted ion
chromatogram.
0
0.5
1.0
1.5
2.0
2.5
3.0
Retention Time [min]
0
100
Rel. Abundance [%]
275
280
285
m/z
278
280
279
281
282
0
50
100
280
281
282
275
280
285
m/z
0
50
100
= System Suitability Test (SST) passed
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Test sites of a Vanquish Core system round robin test using eight different instruments with individual
samples, users, and columns. Each analysis passed the specified SST criteria highlighting the reliability
of the instrument and the ease of use for each user resulting in a successful SST.
Easier method development and routine analysis
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Vanquish Duo for Dual LC
Double your throughput deepen your
sample knowledge and better utilize
your bench space with the Vanquish
Duo for Dual LC workflow. Two
completely independent flow paths for
maximum productivity.
Vanquish Duo for
Tandem LC or LC-MS
Increase your lab’s throughput by 30%
with the Vanquish Duo for Tandem LC
or LC-MS workflow.1 Maximize the
utilization of your optical detector or
mass spectrometer.
Vanquish Duo UHPLC systems
The Thermo Scientific Vanquish Duo UHPLC systems improve
productivity and sample knowledge to deliver a faster return on
investment. The Vanquish Duo UHPLC systems expand the
benefits of our award-winning Vanquish platform with four
workflows (Dual LC, Tandem LC or LC-MS, Transcend Duo LX-2,
and Inverse Gradient). Vanquish Duo UHPLC systems use a
separate second flow path in one integrated system set up by
using Vanquish Flex or Vanquish Horizon modules.
Four workflows. Two flow paths.
One integrated UHPLC solution.
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Vanquish Duo for
Transcend Duo LX-2
Double the throughput of your LC-MS
application with the Vanquish Duo for
Transcend Duo LX-2. Run up to two
applications simultaneously with a
multi-channel LC while benefiting from
the robustness, performance, and ease of
use inherent of the Vanquish LC platform.
Vanquish Duo for
Inverse Gradient LC
Improve your lab’s quantification
capabilities with the Vanquish Duo for
Inverse Gradient, employing the unique
and universal Charged Aerosol Detection,
to quantify all non-volatile and semi-volatile
compounds even when no individual
reference standard is available.
Flow Path 1
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Application
Switch Time
Analytical Gradient
Application 2
Recondi-
tioning
Analytical Gradient
Application 2
Recondi-
tioning
Analytical Gradient
Application 2
Recondi-
tioning
Analytical Gradient
Application 2
Application
Switch Time
Single Channel LC
Flow Path 1
Flow Path 2
Application
Switch Time
Analytical Gradient
Application 2
Analytical Gradient
Application 2
Time savings
Compared to single channel LC
Tandem LC or LC-MS
Time savings
Compared to single channel LC
Flow Path 2
Dual LC
Analytical Gradient
Application 1
Recondi-
tioning
Analytical Gradient
Application 1
Recondi-
tioning
Analytical Gradient
Application 1
Recondi-
tioning
Analytical Gradient
Application 1
Recondi-
tioning
Flow Path 1
Flow Path 2
Dual LC
Analytical Gradient
Application 1
Analytical Gradient
Application 1
Flow Path 1
Flow Path 2
Resulting
MS Signal
Multichannel LC-MS
Analytical Gradient
Application 1
Analytical Gradient
Application 1
Flow Path 1
Recondi-
tioning
Recondi-
tioning
Analytical Gradient
Application 1
Analytical Gradient
Application 1
Flow Path 1
Flow Path 2
Recondi-
tioning
Recondi-
tioning
Inverse Gradient Method — Improved quantification with CAD
Analytical Gradient
Application 1
Analytical Gradient
Application 1
Analytical Gradient
Application 2
Recondi-
tioning
Analytical Gradient
Application 2
Recondi-
tioning
Analytical Gradient
Application 1
Application 1
Analytical Gradient
Application 1
Analytical Gradient
Application 1
Analytical Gradient
Application 1
Analytical Gradient
Application 2
Analytical Gradient
Application 2
Analytical Gradient
Application 2
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Recondi-
tioning
Application 2
Application 1
Application 2
Application 1
Application 2
Application 1
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Powerful fluorescence detection
Fluorescence detection enables high sensitivity detection of
fluorescent compounds with a long-life lamp technology.
Cost-effective, reliable UV-Vis
UV-Vis detection with excellent
sensitivity and linearity is available
to streamline in targeted compound
analysis.
Choosing the right detection technology is key to revealing all the
components of interest in your sample. The Vanquish HPLC and
UHPLC platforms offers a wide range of detection capabilities that
can be easily integrated and combined to fit your methods.
FLD chromatogram of the four aflatoxins: G2, G1, B2 and B1 at a
concentration of 0.9 µg/kg for G2 and B2 and 2.9 µg/kg for G1 and B1.
Industry-leading diode array detection
The industry-leading Thermo Scientific™ Vanquish™
diode array detectors offers the widest linear range and
excellent signal-to noise performance, exceeding that
of variable wavelength detectors. For extremely
demanding applications, increase your detection
sensitivity with the Thermo Scientific™ Diode Array
Detector HL using Thermo Scientific™ LightPipe™ flow
cells. LightPipe technology achieves excellent signal-
to-noise performance with low baseline noise and
drift, and low peak dispersion. Leverage the Thermo
Scientific™ Diode Array Detector FG or CG for the
highest linear range and smooth LC-MS coupling.
Concentration vs. peak height plot of nevirapine with data
points that were considered for calibration (black) and data
points that were eliminated from calibration due to curve
decline (red). Linear calibration with permitted offset and
no weighting.
0
1000
2000
3000
4000
5000
0
400
800
1200
Peak Height [mAU]
Nevirapine concentration [µg/mL]
0.0
0.5
1.0
1.5
2.0
2.5
3.0
3.5
4.0
0.0e0
5.0e5
1.0e6
1.5e6
Retention time [min]
C
oun
ts
G2
G1
B2
B1
Detect the lowest sample quantities
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Uniquely universal charged aerosol detection
The Thermo Scientific™ Vanquish™ charged aerosol
detector is powerful and universal, able to detect
virtually any large and small molecule that lacks
a chromophore, or that poorly ionizes, with
sub-nanogram-level sensitivity and near-uniform
response. The flexibility and performance of charged
aerosol detection is ideal for analytical R&D, while its
simplicity and reproducibility benefit manufacturing
QA/QC applications.
Single quadrupole mass detection
Mass spectrometry gives access to valuable data no
other technology can deliver. The easy to use Thermo
Scientific™ ISQ™ EM single quadrupole mass
spectrometer or the Thermo Scientific™ ISQ™ EC
single quadrupole mass spectrometer integrates with
LC systems for reliable, robust, and easy LC-MS
routine analysis with an extended mass range for
more flexibility. Integrated software allows both
novices and experts to quickly master MS to gain
more insights from every sample.
Unlike mass spectrometry (A) and UV (B), the CAD is able to
measure all analytes in the sample. Mass spectrometry (MS)
requires the analyte to form gas phase ions while response
by a UV detector depends upon the nature of the chromophore.
0
2
4
Time [min]
Retention time [min]
LC-MS TIC positive ionscan m/z 250−1250
Erucamide
Stearic acid
Palmitic
acid
Tinuvin
234
UV at 220 nm
Charged Aerosol Detection
0
0.5
1
1.5
2
Void + Cl-
Propanolol
Ketoprofen
Verapamil
UV at 220 nm
UV at 254 nm
Charged
Aerosol
Detection
The Vanquish platform combines seamlessly with Thermo
Scientific™ mass spectrometers, providing an extra level of
information, confidence and productivity. Mass spectrometry
provides sensitivity and selectivity for your analyses, giving you
more insight into your samples and ability to resolve difficult
separations, including co-eluting peaks, using differing
mass-to-charge (m/z) ratios.
Get better LC connections
Thermo Scientific™ Viper™ fingertight fittings revolutionize the way
UHPLC connections are made. Viper fittings pave the way for
easy setup of virtually dead-volume-free chromatography, even
for your most advanced column configurations. Now you can
enjoy tool-free system fluidics setup and other connections.
Viper fingertight fittings
Thermo Scientific Vanquish system with the Thermo Scientific™ Orbitrap
Exploris™ 480 mass spectrometer.
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Seamlessly integrate MS
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One CDS to do it all
Benefit from the industry-leading, easy-to-use and compliant Chromeleon
CDS with eWorkflow™ procedures for error-free sequence and method setup,
dedicated ePanels for direct instrument control, walk-up Open Access
software, and smart tools to streamline data processing and reporting.
Chromeleon CDS delivers advanced system communication with single-point
intelligent control and functionality, and provides plugins for the Vanquish
UHPLC systems.
Optimize your separations with Thermo Scientific columns
The Vanquish HPLC and UHPLC systems are designed around the
column, the core to every LC separation. Our family of Thermo
Scientific™ Vanquish™ LC columns, partnered with the Vanquish
platform to take advantage of its extended pressure capabilities
and robustness allowing you to optimize your separations faster and
more easily. In addition, all Vanquish LC valves are biocompatible,
have a long-lifespan, and low maintenance, meeting the
performance needs to make LC run robustly and routinely.
Chromeleon CDS for unparalleled Vanquish
UHPLC system control.
Maximize your results
Find out more at
thermofisher.com/liquidchromatography
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Laboratories are constantly asked to do more with less. Built from the ground up,
Thermo Scientific HPLC and UHPLC instruments enable you to raise your productivity
to the next level and give you confidence in your results. Time and cost associated
with staff training are minimized allowing your laboratory to meet ever-increasing
productivity demands, making it faster to bring products to market. With the largest
portfolio of LC solutions, we remain a steadfast and committed partner in your
endeavor to improve the world around us.
LC that takes your productivity to new heights
The collective power
of chromatography
References
1. Thermo Fisher Scientific. TN72203: Tandem UHPLC operation for high-throughput LC-MS peptide
mapping analyses, 2018, Germering, Germany. https://assets.thermofisher.com/TFS-Assets/
CMD/Technical-Notes/tn-72203-tandem-uhplc-peptide-mapping-tn72203-en.pdf
