Operating Guidelines for ChromTech CHIRAL-AGP, CHIRAL-HSA, and CHIRAL-CBH HPLC Columns

Ultron ES-OVM Method Development Protocol Acidic compounds Begin with 0.02 M Phosphate buffer (pH 3.0) No peaks eluted No or low enantioselectivity Add uncharged modifier (MAX 50%)* Increase pH to 4.0 No or low enantioselectivity SEPARATION Increase pH to 4.0…

Supercritical Fluid Chromatography System Nexera™ UC Software for Efficient Method Development LabSolutions™ MD Application News Efficient Scouting of Chiral Separation Conditions Using LabSolutions MD Yusuke Masuda User Benefits  Supercritical fluid chromatography offers quicker separation of chiral compounds.  LabSolutions…

LAAN-A-LC-E271 Application News Supercritical Fluid Chromatography Automated Optimization of Chiral Separation Parameters Using Nexera UC Chiral Screening System L495 No. Chiral compounds contain asymmetric carbons in their molecules and are not superimposable on their mirror images. HPLC has been the…

Basics of Chiral HPLC Definitions Principles Available CSPs Mobile phase types T408109 sigma-aldrich.com The Field of Stereochemistry 1. All isomers have same chemical formula but differ in the arrangement of certain chemical groups in space. 2. Many types of isomers…