Method Migration of a Reversed Phase TFAAcetonitrile Gradient Method From a Binary to a Quaternary System: Impact of Mixing

COMPARISON OF MIXER PERFORMANCE FOR HPLC METHODS UTILIZING TFA GRADIENTS Authors: Lise Gauthier, Kimberly Martin, Elom Pedanou, Paula Hong, and Jennifer Simeone Affiliations: Waters Corporation, 34 Maple Street, Milford, MA 01757 INTRODUCTION Solvent mixing is critical to obtaining optimal LC…

Application Note Improved Sensitivity for Trifluoroacetic Acid Gradients on the Alliance™ iS HPLC Systems Kimberly Martin, Lise Gauthier, Paula Hong Waters Corporation Abstract Given the nature of biomolecules, such as amino acids and peptides, many HPLC reversed phase bioseparations often…

TECHNICAL NOTE 73582 Intelligent UHPLC pump stroke control Ensures sensitive peptide analysis by ripple-free baseline in TFA applications Authors: Giorgia Greco, Dennis Koehler, Christoph Strobl, Markus M. Martin Thermo Fisher Scientific, Germering, Germany Keywords: Baseline ripples, stroke volume, UHPLC, SmartStroke,…

TECHNICAL NOTE 72505 Trifluoroacetic acid performance of the Vanquish Flex Binary UHPLC system Authors Christoph Strobl, Matthias Krajewski, and Remco Swart Thermo Fisher Scientific, Germering, Germany Keywords Trifluoroacetic acid, baseline ripples, mixer volume, stroke volume, flow consistency Goal Mixing ripple…