Vitamins D2 & D3 on a Thermo Scientific™ Acclaim ™ Mixed-Mode WAX-1 Column in HILIC Mode

Fat-Soluble Vitamin Separation Using a Thermo Scientific™ Acclaim™ RSLC PolarAdvantage (PA) Column 40 2 Column: 3 8 6 Dimensions: LC System: 9 5 mAU Mobile Phases: 7 Gradient Time (min): %A: %B: Flow Rate: Temperature: Inj. Volume: Detection: Peaks: 4…

Fat-Soluble Vitamin Separation Using a Thermo Scientific™ Acclaim™ Phenyl-1 Column Column: 60 2 mAU Dimensions: LC System: 3 Mobile Phase: Flow Rate: Temperature: Inj. Volume: Detection: Peaks: 5 4 6 8 7 1 9 0 0 5 10 15 Minutes…

Acclaim mixed-mode WAX-1 Acclaim mixed-mode WCX-1 O– O– R R R R R N N R R N R N R R O C O C O O– C O Acclaim mixed-mode HILIC-1 O– C O O– OH OH N…

Fat-Soluble Vitamin Standards Using a Thermo Scientific™ Acclaim™ PolarAdvantage (PA) Column 22 1 2 Column: Thermo Scientific™ Acclaim™ PA, 3 µm Dimensions: 2.1 × 100 mm Mobile Phase: 97/3 acetonitrile/NH4HCO2, pH 3.4 Flow Rate 0.25 mL/min Temperature: 30 °C Inj.…