Conjugate, clean-up, & characterize ADCs on Unagi & Stunner

Application Note Pharma and Biopharma A streamlined drug-to-antibody ratio determination workflow for intact and deglycosylated antibody-drug conjugates Using the Agilent AssayMAP Bravo and Agilent 6545XT AdvanceBio accurate-mass quadrupole time-of-flight LC/MS system Authors Jerry Han, Zach Van Den Heuvel, and Steve…

WP1621: Multi-Attribute Quantitation of Antibody-Drug Conjugates by LC-MALS Hongjiang (Leo) Liu, Ph.D., and Sophia Kenrick, Ph.D., Wyatt Technology, LLC Summary Liquid chromatography combined with multi-angle light scattering (LC-MALS) is a powerful tool for analyzing antibody-drug conjugates (ADCs), providing a robust…

Agilent BioHPLC columns Characterization of Antibody-Drug Conjugate Critical Quality Attributes Application compendium Contents Introduction3 Featured application notes 2 PLRP-S Polymeric Reversed-Phase Column for LC/MS Separation of mAbs and ADC (5991-7163EN) 5 Intact- and Peptide-Level Characterization of Trastuzumab Emtansine In Vitro…

Analysis of Cysteine-Linked Antibody Drug Conjugates Using Hydrophobic Interaction Chromatography on the Agilent 1260 Infinity II Bio-inert LC Application Note Biologics and Biosimilars Author Abstract Sonja Schneider Hydrophobic interaction chromatography (HIC) is frequently used for the Agilent Technologies, Inc. determination…