Method considerations for therapeutic ASO RNA analysis. Adducts and insource impurity generation

Oligonucleotide analysis Green ion pair and HFIP free method for ASO RNA analysis with GLP compliant automated data handling Ulrik H. Mistarz1, Marcus Hoffmann2, Heiko Herrmann2, Alexander Schwahn3, Simon Szwandt4 Ken Cook4 1Thermo Fisher Scientific, Denmark, 2Thermo Fisher Scientific, Germany,…

Oligonucleotide analysis Detailed study into ASO impurity analysis, lessons learned, and myths dispelled while moving to compliant platform methods Ulrik H. Mistarz1, Marcus Hoffmann2, Heiko Herrmann2, Richard Law3, Alexander Schwahn4, Ken Cook5 1Thermo Fisher Scientific, Denmark, 2Thermo Fisher Scientific, Germany,…

Cation exchange coupled to high resolution mass spectrometry for the analytical characterization of phosphorodiamidate morpholino oligomers K Cook[a];U H Mistarz[b]; S Millán-Martín[c]; S Carillo[c]; J Bones[c] [a]Thermo Fisher Scientific, United Kingdom; [b]Thermo Fisher Scientific, Denmark; [c]National Institute for Bioprocessing Research…

Oligonucleotides Capillary flow ion pair reversed-phase separation for very sensitive oligonucleotide LC-HRMS analysis and characterisation Ulrik H. Mistarz1, Shanhua Lin2, Shane L. Bechler2, Brandon H. Robson2, Ken Cook3 1) Thermo Fisher Scientific, Allerød, Denmark, 2) Thermo Fisher Scientific, Sunnyvale, CA…