An improved HPAE-PAD method for glycoprotein monosaccharide determination

TECHNICAL NOTE 72225 Glycoprotein monosaccharide analysis using HPAE-PAD with manually prepared eluent Authors Sachin Patil and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Goal To present an accurate method of determining monosaccharides in glycoproteins using high-performance anion-exchange chromatography with…

Lipika Basumallick and Jeffrey Rohrer, Thermo Fisher Scientific, Sunnyvale, CA, USA Key Words Carbohydrate Analysis, High Throughput, Bovine Fetuin, Human Immunoglobulin (IgG), Gold on PTFE Disposable Electrode Goal This study is intended as a guide for investigators interested in validating…

APPLICATION BRIEF 72720 A glycopeptide standard for improving glycoprotein hydrolysis reaction yield for accurate monosaccharide content determination Authors Introduction Sachin Patil and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA High-performance anion-exchange chromatography with pulsed amperometric detection (HPAE-PAD) is a well-established…

TECHNICAL NOTE 73348 Carbohydrate determinations by HPAE-PAD using a PdH reference electrode Authors: Manali Aggrawal and Jeffrey Rohrer Thermo Fisher Scientific, Sunnyvale, CA Keywords: ICS-6000, Dionex CarboPac PA210-4µm column, Dionex CarboPac PA20-4µm column, monosaccharides, honey, sugars, glycoprotein Goal To demonstrate…