Oligo Purity Analysis and Sequence Confirmation by LC/MS without Ion Pairing Reagents — Sample to reports in about 5 minutes

Application Note BioPharma MS/MS Oligonucleotide Sequencing Using LC/Q-TOF with HILIC Chromatography Authors Guannan Li and Peter Rye Agilent Technologies, Inc. Introduction Oligonucleotides are commonly analyzed by liquid chromatography/mass spectrometry (LC/MS) in negative ion polarity mode using ion-pairing reversed‑phase (IP‑RP) methods.…

Poster Reprint ASMS 2026 MP 536 Comprehensive characterization of oligonucleotides and related impurities using advanced LC/Q-TOF analytical workflows Saikat Banerjee, Preeti Bharatiya, Ashish Pargaonkar, Vivek Dhyani; Agilent technologies, India Introduction Therapeutic oligonucleotides are synthetic, short nucleic acid sequences designed to…

Application Note Pharma & Biopharma Oligonucleotides Oligonucleotide Characterization by Agilent 1290 Infinity II Bio LC and 6545XT AdvanceBio LC/Q-TOF Oligonucleotide identity, impurities analysis and sequence determination using BioConfirm 12.0 target plus impurities (TPI) and sequence confirmation workflows Authors Introduction Bian…

Application Note BioPharma MS1 Oligonucleotide Characterization Using LC/Q-TOF with HILIC Chromatography Authors Introduction Peter Rye and Cody Schwarzer Agilent Technologies, Inc. The synthesis of RNA and DNA oligonucleotides (oligos) is an iterative process that, despite highly optimized chemistry, results in…