Characterization of carbamoylated lysine in a therapeutic recombinant protein using top-down electron fragmentation

Transformative Workflows for Protein Characterization: ECD and Multidimensional Separations Expert Insights Sponsored by Expert Insights Contents Introduction 3 The Role of Electron Capture Dissociation in Biomolecular Analysis Adapted from Cooper, et al. 4 Offline Coupling of Hydrophobic Interaction Chromatography– Capillary…

Application Note Biopharma Identification of Amino Acid Isomers Using Electron Capture Dissociation in the Agilent 6545XT AdvanceBio LC/Q-TOF System Authors Rachel Franklin and Joseph Meeuwsen Agilent Technologies, Inc. Abstract Accurately determining the amino acid sequence is crucial for understanding a…

Application Note Biopharma Top-Down Sequence Analysis of Intact Proteins Using an Agilent AdvanceBio 6545XT LC/Q-TOF with ExD Authors Rachel Franklin and Mike Hare Agilent Technologies, Inc. Abstract Detailed proteoform characterization is crucial for understanding disease mode of action (MOA) and…

Application Note Biopharma Comprehensive Characterization of Multiple GLP-1 Analogs Using an Agilent 6545XT AdvanceBio LC/Q-TOF with electron capture dissociation and ExDViewer software Authors Rachel Franklin, Mike Hare, Thomas Walker, and Joseph Meeuwsen Agilent Technologies, Inc. Abstract GLP-1 analogs are synthetic…