Determination of the Phosphate Content of Phosphorylated Proteins

Application Note 99 Peptide Mapping of Ovalbumin Using ReversedPhase High-Performance Liquid Chromatography and Prediction of Phosphopeptide Elution INTRODUCTION Protein variants arise from post-translational modifications, such as glycosylation, oxidation, and phosphorylation. Variants of proteins produced for medicinal purposes can occur during…

Application Note 202 High Performance Anion-Exchange Chromatography with Pulsed Amperometric Detection (HPAE-PAD) Analysis of Mannose-6-Phosphate Introduction D-Mannose-6-phosphate (M-6-P) is a terminal monosaccharide of asparagine-linked oligosaccharides that is an important intermediate in glycoprotein production and, when incorporated in the glycoprotein's final…

columns Ion-Exchange Columns for Characterization and Quality Control Assessment of Closely Related Protein Variants: • • • • • Sequence Truncation Phosphorylation Sialylation Deamidation ProPac® Ion Exchange Columns for Protein Analysis Unique Chemistry for High-Resolution Analysis of Proteins with Small…

Application Note 200 Direct Determination of Cyanate in a Urea Solution and a Urea-Containing Protein Buffer INTRODUCTION Urea is commonly used in protein purification, including large scale purification of recombinant proteins for commercial purposes, and in recombinant protein manufacturing to…