Automated Workflow for Proteoforms Characterization and Quantification of Intact Monoclonal Antibodies by CEX-MS

APPLICATION NOTE 73884 Monoclonal antibody mass confirmation using intact protein deconvolution for a compliance-ready manufacturing environment Authors: Kristina Srzentić1, Kai Scheffler2, Royston Quintyn3, Artem Akhmetov4, Jennifer Sutton1 Thermo Fisher Scientific, 1 Cambridge, Massachusetts, US 2 Germering, Germany 3 West Palm…

HPLC Columns LC-MS analysis of intact and subunit-level mAb enabled by a novel monodisperse supermacroporous reversed-phase platform Audrius Žvirblis1, Odeta Dembovskytė1, Ke Ma2, Christof Mitterer3, Mauro De Pra4 and Frank Steiner5 1Thermo Fisher Scientific, Vilnius, Lithuania, 2Thermo Fisher Scientific, CA,…

Biopharma Protein solutions eBook Protein therapeutics: At a glance Protein therapeutics are a type of biotherapeutics derived from genetically engineered human proteins. They offer several advantages over small-molecule drugs, allowing for more precise targeting of complex functions. Compared to chemical…

Application note | 003310 Biopharma Monitoring of multiple quality attributes of intact monoclonal antibodies from bioreactors by switchable 2D-LC-MS Authors Application benefits Maria Grübner , Kai Scheffler , 1 1 • Heart-cut 2D-LC with protein A affinity chromatography (ProA) of…