Improved Separation Performance of Monoclonal Antibody and Antibody Drug Conjugates Using Waters XBridge Premier Protein SEC 250 Å, 2.5 μm Column

[ APPLICATION NOTEBOOK ] SIZE-EXCLUSION CHROMATOGRAPHY (SEC) OF PEPTIDES, PROTEINS, AND AAVs [ SIZE-EXCLUSION CHROMATOGRAPHY ] [ SIZE-EXCLUSION CHROMATOGRAPHY ] Introduction Gel-permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel-filtration chromatography (GFC) are commonly used terms to describe a liquid chromatographic…

Application Note MaxPeak Premier Protein SEC 250 Å Column Lifetimes at Physiological pH for Polysorbate (Tween) Formulated Biosimilar Monoclonal Antibodies Stephan M. Koza, Hua Yang, Ying Qing Yu Waters Corporation Abstract Self-associated or aggregated protein impurities in therapeutics is a…

Application Note USP Monograph mAb SEC Method Robustness on the XBridge Premier Protein SEC Column Hua Yang, Stephan M. Koza, Ying Qing Yu Waters Corporation Abstract Size-exclusion chromatography (SEC) has been widely used for size-based impurity analysis. We show here…

Application Note Rapid Size Variant Analysis of Monoclonal Antibodies Using UPLC™ and HPLC Compatible MaxPeak™ Premier Protein SEC Columns Stephan M. Koza, Ying Qing Yu Waters Corporation Abstract High-throughput and rapid size-exclusion chromatography (SEC) methods can be useful to support…