Complete de novo sequencing of a human immunoglobulin G using multiple enzyme digests and LC-ESI-QTOF-MS/MS analysis

CUSTOMER APPLICATION NOTE 73826 Peptide mapping of challenging monoclonal antibodies Authors: Dan Bach Kristensen1, Trine Meiborg Sloth1, Martin Ørgaard1, Krisztina Radi2 Symphogen, Ballerup, Denmark Thermo Fisher Scientific, Hemel Hempstead, UK 1 2 Keywords: Peptide mapping, monoclonal antibody, mAb, post-translational modifications,…

ApplicAtion SolutionS for BiophArmAceuticAlS A Focus on Protein Therapeutics Introduction: Key Challenges in Biopharmaceutical Characterization .............................................................................................5 7 PROTEIN mass aNalysIs comprehensive and routine characterization of proteins and peptides by lc/mS and lc/mS e ...............................................................9 characterization of an igG1 monoclonal Antibody…

ASMS 2023 - THP 378 Real-time de novo sequencing of peptide antigens using Bruker ProteoScape™ for 'Run & Done' 4D-immunopeptidomics A 5000 4500 4000 3500 3000 2500 2000 1500 1000 500 0 1Monash University, Clayton, Australia, 2Rapid Novor Inc., Kitchener,…

Extensive LC-Top-Down MS Sequence Confirmation of the NISTmAb Reference Material 8671 Characterization of therapeutic antibodies aims on primary sequence validation, localization of modifications such as glycan profiles and the determination of disulfide bond status of the target molecule. Introduction Typically,…