AN INTEGRATED WORKFLOW FOR AUTOMATIC MAPPING OF DISULFIDE LINKAGES OF THERAPEUTIC PROTEINS USING HIGH-RESOLUTION LCMS, ETD FRAGMENTATION AND TARGETED INFORMATICS

[ APPLICATION NOTE ] Automated Disulfide Bond Mapping, and Comparing Innovator and Biosimilar mAbs Using UNIFI Henry Shion, Min Du, and Ying Qing Yu Waters Corporation, Milford, MA, USA APPLICATION BENEFITS This application note describes a UPLC-MS INTRODUCTION E Disulfide…

BIOPHARMACEUTICAL PLATFORM SOLUTION Key Applications TABLE OF CONTENTS Biopharmaceutical Platform Solution with UNIFI: Key Applications Intact Protein MS Analysis I Peptide Mapping I Released Glycan Analysis I Bioseparations Biotherapeutic proteins – such as monoclonal antibodies (mAbs), biosimilars, and antibody drug…

ApplicAtion SolutionS for BiophArmAceuticAlS A Focus on Protein Therapeutics Introduction: Key Challenges in Biopharmaceutical Characterization .............................................................................................5 7 PROTEIN mass aNalysIs comprehensive and routine characterization of proteins and peptides by lc/mS and lc/mS e ...............................................................9 characterization of an igG1 monoclonal Antibody…

Poster Note 64 8 0 8 Disulfide Bond Identification of Biotherapeutic Proteins Using Various Fragmentation Techniques Available on an Orbitrap Fusion Tribrid Mass Spectrometer Disulfide Disulfide bond bond identification identification of of biotherapeutic biotherapeutic prot prot Fusion Fusion Tribrid Tribrid…