Mapping IgG Subunit Glycoforms Using HILIC and a Wide-Pore Amide Stationary Phase

HILIC Glycopeptide Mapping with a Wide-Pore Amide Stationary Phase Matthew A. Lauber and Stephan M. Koza Waters Corporation, Milford, MA, USA A P P L I C AT I O N B E N E F I T S ■■…

Waters Application Notes Glycans There are a variety of complementary techniques practiced to get the complete story about a glycoprotein. Each technique varies in complexity and provides a different layer of information. Method complexity This application notebook highlights a body…

MAIN MENU TABLE OF CONTENTS [ APPLICATIONS NOTEBOOK ] BIOSEPARATIONS Tools, Techniques, and Insights into Biopharmaceutical Analysis INT RODUCTION Biopharmaceuticals have emerged as a dominant class due to their specificity and efficacy. T he production of biopharmaceuticals, however, is distinctly…

Using Hydrophilic Interaction Chromatography for Heightened Product Characterization to Overcome Challenges with Hydrophobic Monoclonal Antibodies and Antibody Drug Conjugates Jacquelynn Smith,1 Matthew A. Lauber,3 Stephan M. Koza,3 Erin E. Chambers,3 Jason C. Rouse,2 and Olga V. Friese1 Biotherapeutics Pharm. Sci.,…