Measuring the Glycan Occupancy of Intact mAbs using HILIC and Detection by Intrinsic Fluorescence

Waters Application Notes Glycans There are a variety of complementary techniques practiced to get the complete story about a glycoprotein. Each technique varies in complexity and provides a different layer of information. Method complexity This application notebook highlights a body…

Developing High Resolution HILIC Separations of Intact Glycosylated Proteins Using a Wide-Pore Amide-Bonded Stationary Phase Matthew A. Lauber, Scott A. McCall, Bonnie A. Alden, Pamela C. Iraneta, and Stephan M. Koza Waters Corporation, Milford, MA, USA A P P L…

[ GLYC AN ANALYSIS SOLUTIONS ] The Next Generation of Glycan Sample Preparation and Analysis [ GLYC AN ANALYSIS SOLUTIONS ] What if... identifying WHERE glycans are present on a glycoprotein becomes part of your routine workflow? 1 [ GLYC…

MAIN MENU TABLE OF CONTENTS [ APPLICATIONS NOTEBOOK ] BIOSEPARATIONS Tools, Techniques, and Insights into Biopharmaceutical Analysis INT RODUCTION Biopharmaceuticals have emerged as a dominant class due to their specificity and efficacy. T he production of biopharmaceuticals, however, is distinctly…