Characterization of EPO N-Glycans using RapiFluor-MS and HILIC Profiling

Comprehensive Characterization of the N and O-Linked Glycosylation of a Recombinant Human EPO Matthew A. Lauber, Stephan M. Koza, and Erin E. Chambers Waters Corporation, Milford, MA, USA A P P L I C AT I O N B E…

Waters Application Notes Glycans There are a variety of complementary techniques practiced to get the complete story about a glycoprotein. Each technique varies in complexity and provides a different layer of information. Method complexity This application notebook highlights a body…

MAIN MENU TABLE OF CONTENTS [ APPLICATIONS NOTEBOOK ] BIOSEPARATIONS Tools, Techniques, and Insights into Biopharmaceutical Analysis INT RODUCTION Biopharmaceuticals have emerged as a dominant class due to their specificity and efficacy. T he production of biopharmaceuticals, however, is distinctly…

Enhancing the Peak Capacity of High Molecular Weight N-Glycan HILIC Separations with a Wide-Pore Amide Bonded Stationary Phase Matthew A. Lauber and Stephan M. Koza G OA L Wide-pore glycoprotein BEH amide, 300Å, 1.7 µm To demonstrate the enhanced resolving…